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Literature summary extracted from

  • Dammeyer, T.; Frankenberg-Dinkel, N.
    Insights into phycoerythrobilin biosynthesis point toward metabolic channeling (2006), J. Biol. Chem., 281, 27081-27089.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.7.2 expressed using a tac promoter-driven N-terminal GST fusion protein Synechococcus sp.
1.3.7.2 into pGEX-6P-3 vector for N-terminal fusion with glutathione S-transferase and transformed in Escherichia coli BL21(lambdaDE3) cells Microchaete diplosiphon
1.3.7.3 expressed using a tac promoter-driven N-terminal GST fusion protein Synechococcus sp.
1.3.7.3 into pASK-IBA45+ and transformed in Escherichia coli DH10B cells Microchaete diplosiphon

Organism

EC Number Organism UniProt Comment Textmining
1.3.7.2 Microchaete diplosiphon Q6UR88
-
-
1.3.7.2 Microchaete diplosiphon Fd33 Q6UR88
-
-
1.3.7.2 Synechococcus sp.
-
-
-
1.3.7.2 Synechococcus sp. WH8020
-
-
-
1.3.7.3 Microchaete diplosiphon Q6UR87
-
-
1.3.7.3 Microchaete diplosiphon Fd33 Q6UR87
-
-
1.3.7.3 Synechococcus sp.
-
-
-
1.3.7.3 Synechococcus sp. WH8020
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.7.2 by two affinity chromatography steps to 90% purity and a third gel filtration step Synechococcus sp.
1.3.7.2 by two affinity chromatography steps to 90% purity and a third gel filtration step Microchaete diplosiphon
1.3.7.3 by gel filtration Microchaete diplosiphon
1.3.7.3 by two affinity chromatography steps to 90% purity and a third gel filtration step Synechococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.7.2 15,16-dihydrobiliverdin + reduced ferredoxin
-
 Microchaete diplosiphon phycoerythrobilin + oxidized ferredoxin
-
 r
1.3.7.2 15,16-dihydrobiliverdin + reduced ferredoxin
-
 Microchaete diplosiphon Fd33 phycoerythrobilin + oxidized ferredoxin
-
 r
1.3.7.2 biliverdin IXa + reduced ferredoxin
-
 Synechococcus sp. 15,16-dihydrobiliverdin + oxidized ferredoxin
-
 r
1.3.7.2 biliverdin IXa + reduced ferredoxin
-
 Microchaete diplosiphon 15,16-dihydrobiliverdin + oxidized ferredoxin
-
 r
1.3.7.2 biliverdin IXa + reduced ferredoxin
-
 Synechococcus sp. WH8020 15,16-dihydrobiliverdin + oxidized ferredoxin
-
 r
1.3.7.2 biliverdin IXa + reduced ferredoxin
-
 Microchaete diplosiphon Fd33 15,16-dihydrobiliverdin + oxidized ferredoxin
-
 r
1.3.7.2 additional information does not convert 3E-phycocyanobilin Microchaete diplosiphon ?
-
 ?
1.3.7.2 additional information does not convert phycocyanobilin Synechococcus sp. ?
-
 ?
1.3.7.2 additional information does not convert phycocyanobilin Synechococcus sp. WH8020 ?
-
 ?
1.3.7.2 additional information does not convert 3E-phycocyanobilin Microchaete diplosiphon Fd33 ?
-
 ?
1.3.7.2 phytochromobilin + biliverdin IXa + reduced ferredoxin
-
 Synechococcus sp. phycoerythrobilin
-
 r
1.3.7.2 phytochromobilin + biliverdin IXa + reduced ferredoxin
-
 Synechococcus sp. WH8020 phycoerythrobilin
-
 r
1.3.7.3 15,16-dihydrobiliverdin + reduced ferredoxin
-
 Synechococcus sp. (3Z)-phycoerythrobilin + oxidized ferredoxin
-
 r
1.3.7.3 15,16-dihydrobiliverdin + reduced ferredoxin
-
 Microchaete diplosiphon (3Z)-phycoerythrobilin + oxidized ferredoxin
-
 r
1.3.7.3 15,16-dihydrobiliverdin + reduced ferredoxin
-
 Synechococcus sp. WH8020 (3Z)-phycoerythrobilin + oxidized ferredoxin
-
 r
1.3.7.3 15,16-dihydrobiliverdin + reduced ferredoxin
-
 Microchaete diplosiphon Fd33 (3Z)-phycoerythrobilin + oxidized ferredoxin
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.3.7.2 PebA
-
 Synechococcus sp.
1.3.7.2 PebA
-
 Microchaete diplosiphon
1.3.7.3 PebB
-
 Synechococcus sp.
1.3.7.3 PebB
-
 Microchaete diplosiphon

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.7.2 7.5
-
-
 Microchaete diplosiphon

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.7.2 Ferredoxin
-
 Synechococcus sp.
1.3.7.2 Ferredoxin
-
 Microchaete diplosiphon
1.3.7.2 additional information insensitivity toward metal chelators like 0.01 mM EDTA, 0.005 mM o-phenanthroline, or 0.005 mM 2,2'-dipyridyl Microchaete diplosiphon
1.3.7.2 NADP+
-
 Synechococcus sp.
1.3.7.2 NADP+
-
 Microchaete diplosiphon
1.3.7.3 Ferredoxin
-
 Synechococcus sp.
1.3.7.3 Ferredoxin
-
 Microchaete diplosiphon
1.3.7.3 NADP+
-
 Synechococcus sp.
1.3.7.3 NADP+
-
 Microchaete diplosiphon