EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.3.9 | additional information | overexpression of recombinant UDP-GlcNAc 2-epimerase/ManNAc 6-kinase, GNE, in HEK AD293 cells up-regulates GM3 synthase, while GNE down-regulation by RNA interference or exogenous GM3 and GD3 down-regulates the GM3 synthase | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.60 | expressed in Escherichia coli strain DH5alpha | Homo sapiens |
5.1.3.14 | expression in Escherichia coli | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.3.9 | additional information | overexpression of recombinant UDP-GlcNAc 2-epimerase/ManNAc 6-kinase, GNE, in HEK AD293 cells up-regulates GM3 synthase, while GNE down-regulation by RNA interference or exogenous GM3 and GD3 down-regulates the GM3 synthase | Homo sapiens |
5.1.3.14 | R266Q | GNE mutants are created by site-directed mutagenesis with the mutagenic oligonucleotides 5'-GGTTCGAGTGATGCAGAAGAAGGGCATTGAGCA-3' for the R266Q sialuria mutations (where the site of mutation is underlined) through PCR-like amplification with Pfu polymerase. | Homo sapiens |
5.1.3.14 | R266W | GNE mutants are created by site-directed mutagenesis with the mutagenic oligonucleotides 5'-GGTTCGAGTGATGTGGAAGAAGGGCATTGAGCA-3' for the R266W sialuria mutations (where the site of mutation is underlined) through PCR-like amplification with Pfu polymerase. | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.3.9 | additional information | overexpression of recombinant UDP-GlcNAc 2-epimerase/ManNAc 6-kinase, GNE, in HEK AD293 cells up-regulates GM3 synthase, while GNE down-regulation by RNA interference or exogenous GM3 and GD3 down-regulates the GM3 synthase | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.3.8 | additional information | Homo sapiens | overexpression of recombinant UDP-GlcNAc 2-epimerase/ManNAc 6-kinase in human embryonic kidney (HEK AD293) cells leads to an increase in mRNA levels for ST3Gal5 (GM3 synthase) and ST8Sia1 (GD3 synthase) as well as the biosynthetic products of these sialyltransferases, the GM3 and GD3 gangliosides. Down-regulation of UDP-GlcNAc 2-epimerase/ManNAc 6-kinase by RNA interference methods has the opposite, but consistent, effect of lowering ST3Gal5 and ST8Sia1 mRNAs and reducing GM3 and GD3 levels | ? | - |
? | |
2.4.3.9 | CMP-N-acetylneuraminate + lactosylceramide | Homo sapiens | metabolic flux and expression levels of enzymes involved in the sialic acid biosynthetic pathway, overview | CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosyl-ceramide | - |
? | |
2.4.3.9 | additional information | Homo sapiens | overexpression of recombinant UDP-GlcNAc 2-epimerase/ManNAc 6-kinase, GNE, in HEK AD293 cells up-regulates GM3 synthase, while GNE down-regulation by RNA interference or exogenous GM3 and GD3 down-regulates the GM3 synthase | ? | - |
? | |
5.1.3.14 | UDP-GlcNAc | Homo sapiens | sialic acid biosynthetic pathway | ManNAc + UDP | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.3.8 | Homo sapiens | - |
- |
- |
2.4.3.9 | Homo sapiens | - |
- |
- |
2.7.1.60 | Homo sapiens | - |
- |
- |
5.1.3.14 | Homo sapiens | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.3.8 | HEK AD293 cell | - |
Homo sapiens | - |
2.4.3.9 | HEK-AD293 cell | - |
Homo sapiens | - |
2.7.1.60 | HEK-293 cell | - |
Homo sapiens | - |
5.1.3.14 | kidney cell line | human epithel, HEK AD293 | Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.3.9 | additional information | - |
analysis of cell surface and total levels of GM3 in wild-type and transfected cells | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.3.8 | additional information | overexpression of recombinant UDP-GlcNAc 2-epimerase/ManNAc 6-kinase in human embryonic kidney (HEK AD293) cells leads to an increase in mRNA levels for ST3Gal5 (GM3 synthase) and ST8Sia1 (GD3 synthase) as well as the biosynthetic products of these sialyltransferases, the GM3 and GD3 gangliosides. Down-regulation of UDP-GlcNAc 2-epimerase/ManNAc 6-kinase by RNA interference methods has the opposite, but consistent, effect of lowering ST3Gal5 and ST8Sia1 mRNAs and reducing GM3 and GD3 levels | Homo sapiens | ? | - |
? | |
2.4.3.9 | CMP-N-acetylneuraminate + lactosylceramide | - |
Homo sapiens | CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosyl-ceramide | - |
? | |
2.4.3.9 | CMP-N-acetylneuraminate + lactosylceramide | metabolic flux and expression levels of enzymes involved in the sialic acid biosynthetic pathway, overview | Homo sapiens | CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosyl-ceramide | - |
? | |
2.4.3.9 | additional information | overexpression of recombinant UDP-GlcNAc 2-epimerase/ManNAc 6-kinase, GNE, in HEK AD293 cells up-regulates GM3 synthase, while GNE down-regulation by RNA interference or exogenous GM3 and GD3 down-regulates the GM3 synthase | Homo sapiens | ? | - |
? | |
2.7.1.60 | ATP + N-acetyl-D-mannosamine | - |
Homo sapiens | ADP + N-acetyl-D-mannosamine 6-phosphate | - |
? | |
5.1.3.14 | UDP-GlcNAc | sialic acid biosynthetic pathway | Homo sapiens | ManNAc + UDP | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.3.8 | GD3 synthase | - |
Homo sapiens |
2.4.3.8 | ST8Sia1 | - |
Homo sapiens |
2.4.3.9 | GM3 synthase | - |
Homo sapiens |
2.4.3.9 | ST3Gal5 | - |
Homo sapiens |
2.7.1.60 | GNE | - |
Homo sapiens |
2.7.1.60 | UDP-GlcNAc 2-epimerase/ManNAc 6-kinase | - |
Homo sapiens |
5.1.3.14 | UDP-GlcNAc 2-epimerase | - |
Homo sapiens |