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Literature summary extracted from

  • Wang, Z.; Sun, Z.; Li, A.V.; Yarema, K.J.
    Roles for UDP-GlcNAc 2-epimerase/ManNAc 6-kinase outside of sialic acid biosynthesis: modulation of sialyltransferase and BiP expression, GM3 and GD3 biosynthesis, proliferation, and apoptosis, and ERK1/2 phosphorylation (2006), J. Biol. Chem., 281, 27016-27028.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.3.9 additional information overexpression of recombinant UDP-GlcNAc 2-epimerase/ManNAc 6-kinase, GNE, in HEK AD293 cells up-regulates GM3 synthase, while GNE down-regulation by RNA interference or exogenous GM3 and GD3 down-regulates the GM3 synthase Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.60 expressed in Escherichia coli strain DH5alpha Homo sapiens
5.1.3.14 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.3.9 additional information overexpression of recombinant UDP-GlcNAc 2-epimerase/ManNAc 6-kinase, GNE, in HEK AD293 cells up-regulates GM3 synthase, while GNE down-regulation by RNA interference or exogenous GM3 and GD3 down-regulates the GM3 synthase Homo sapiens
5.1.3.14 R266Q GNE mutants are created by site-directed mutagenesis with the mutagenic oligonucleotides 5'-GGTTCGAGTGATGCAGAAGAAGGGCATTGAGCA-3' for the R266Q sialuria mutations (where the site of mutation is underlined) through PCR-like amplification with Pfu polymerase. Homo sapiens
5.1.3.14 R266W GNE mutants are created by site-directed mutagenesis with the mutagenic oligonucleotides 5'-GGTTCGAGTGATGTGGAAGAAGGGCATTGAGCA-3' for the R266W sialuria mutations (where the site of mutation is underlined) through PCR-like amplification with Pfu polymerase. Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.3.9 additional information overexpression of recombinant UDP-GlcNAc 2-epimerase/ManNAc 6-kinase, GNE, in HEK AD293 cells up-regulates GM3 synthase, while GNE down-regulation by RNA interference or exogenous GM3 and GD3 down-regulates the GM3 synthase Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.3.8 additional information Homo sapiens overexpression of recombinant UDP-GlcNAc 2-epimerase/ManNAc 6-kinase in human embryonic kidney (HEK AD293) cells leads to an increase in mRNA levels for ST3Gal5 (GM3 synthase) and ST8Sia1 (GD3 synthase) as well as the biosynthetic products of these sialyltransferases, the GM3 and GD3 gangliosides. Down-regulation of UDP-GlcNAc 2-epimerase/ManNAc 6-kinase by RNA interference methods has the opposite, but consistent, effect of lowering ST3Gal5 and ST8Sia1 mRNAs and reducing GM3 and GD3 levels ?
-
?
2.4.3.9 CMP-N-acetylneuraminate + lactosylceramide Homo sapiens metabolic flux and expression levels of enzymes involved in the sialic acid biosynthetic pathway, overview CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosyl-ceramide
-
?
2.4.3.9 additional information Homo sapiens overexpression of recombinant UDP-GlcNAc 2-epimerase/ManNAc 6-kinase, GNE, in HEK AD293 cells up-regulates GM3 synthase, while GNE down-regulation by RNA interference or exogenous GM3 and GD3 down-regulates the GM3 synthase ?
-
?
5.1.3.14 UDP-GlcNAc Homo sapiens sialic acid biosynthetic pathway ManNAc + UDP
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.8 Homo sapiens
-
-
-
2.4.3.9 Homo sapiens
-
-
-
2.7.1.60 Homo sapiens
-
-
-
5.1.3.14 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.8 HEK AD293 cell
-
Homo sapiens
-
2.4.3.9 HEK-AD293 cell
-
Homo sapiens
-
2.7.1.60 HEK-293 cell
-
Homo sapiens
-
5.1.3.14 kidney cell line human epithel, HEK AD293 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.3.9 additional information
-
analysis of cell surface and total levels of GM3 in wild-type and transfected cells Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.8 additional information overexpression of recombinant UDP-GlcNAc 2-epimerase/ManNAc 6-kinase in human embryonic kidney (HEK AD293) cells leads to an increase in mRNA levels for ST3Gal5 (GM3 synthase) and ST8Sia1 (GD3 synthase) as well as the biosynthetic products of these sialyltransferases, the GM3 and GD3 gangliosides. Down-regulation of UDP-GlcNAc 2-epimerase/ManNAc 6-kinase by RNA interference methods has the opposite, but consistent, effect of lowering ST3Gal5 and ST8Sia1 mRNAs and reducing GM3 and GD3 levels Homo sapiens ?
-
?
2.4.3.9 CMP-N-acetylneuraminate + lactosylceramide
-
Homo sapiens CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosyl-ceramide
-
?
2.4.3.9 CMP-N-acetylneuraminate + lactosylceramide metabolic flux and expression levels of enzymes involved in the sialic acid biosynthetic pathway, overview Homo sapiens CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosyl-ceramide
-
?
2.4.3.9 additional information overexpression of recombinant UDP-GlcNAc 2-epimerase/ManNAc 6-kinase, GNE, in HEK AD293 cells up-regulates GM3 synthase, while GNE down-regulation by RNA interference or exogenous GM3 and GD3 down-regulates the GM3 synthase Homo sapiens ?
-
?
2.7.1.60 ATP + N-acetyl-D-mannosamine
-
Homo sapiens ADP + N-acetyl-D-mannosamine 6-phosphate
-
?
5.1.3.14 UDP-GlcNAc sialic acid biosynthetic pathway Homo sapiens ManNAc + UDP
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.3.8 GD3 synthase
-
Homo sapiens
2.4.3.8 ST8Sia1
-
Homo sapiens
2.4.3.9 GM3 synthase
-
Homo sapiens
2.4.3.9 ST3Gal5
-
Homo sapiens
2.7.1.60 GNE
-
Homo sapiens
2.7.1.60 UDP-GlcNAc 2-epimerase/ManNAc 6-kinase
-
Homo sapiens
5.1.3.14 UDP-GlcNAc 2-epimerase
-
Homo sapiens