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Literature summary extracted from

  • Garg, R.P.; Gonzalez, J.M.; Parry, R.J.
    Biochemical characterization of VlmL, a Seryl-tRNA synthetase encoded by the valanimycin biosynthetic gene cluster (2006), J. Biol. Chem., 281, 26785-26791.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.11 gene serS, phylogenetic analysis Streptomyces coelicolor
6.1.1.11 gene serS2, phylogenetic analysis Streptomyces avermitilis
6.1.1.11 genes vlmL and svsR, DNA and amino acid sequence determination and analysis from strain MG456-hF10, phylogenetic analysis, overexpression of soluble VlmL and SvsR in Escherichia coli strain BL21(DE3) Streptomyces viridifaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.11 additional information
-
 additional information kinetics Streptomyces viridifaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.11 Mg2+
-
 Streptomyces coelicolor
6.1.1.11 Mg2+
-
 Streptomyces avermitilis
6.1.1.11 Mg2+
-
 Streptomyces viridifaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.11 ATP + L-serine + tRNASer Streptomyces coelicolor
-
 AMP + diphosphate + L-seryl-tRNASer
-
 r
6.1.1.11 ATP + L-serine + tRNASer Streptomyces avermitilis
-
 AMP + diphosphate + L-seryl-tRNASer
-
 r
6.1.1.11 ATP + L-serine + tRNASer Streptomyces viridifaciens
-
 AMP + diphosphate + L-seryl-tRNASer
-
 r
6.1.1.11 additional information Streptomyces viridifaciens SerRS represents the housekeeping seryl-tRNA synthetase in the organism, one role of protein VlmL in valanimycin biosynthesis is to produce seryl-tRNA, which is then utilized for a subsequent step in the biosynthetic pathway, biosynthetic pathway for the antibiotic valanimycin, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.11 Streptomyces avermitilis Q82KS8 gene serS2
-
6.1.1.11 Streptomyces coelicolor Q9ZBX1 gene serS
-
6.1.1.11 Streptomyces viridifaciens Q84F26 a valanimycin producing organism, strain MG456-hF10, genes vlmL and svsR, the latter is a housekeeping gene, gene SerRS
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.11 ATP + L-serine + tRNASer
-
 Streptomyces coelicolor AMP + diphosphate + L-seryl-tRNASer
-
 r
6.1.1.11 ATP + L-serine + tRNASer
-
 Streptomyces avermitilis AMP + diphosphate + L-seryl-tRNASer
-
 r
6.1.1.11 ATP + L-serine + tRNASer
-
 Streptomyces viridifaciens AMP + diphosphate + L-seryl-tRNASer
-
 r
6.1.1.11 additional information SerRS represents the housekeeping seryl-tRNA synthetase in the organism, one role of protein VlmL in valanimycin biosynthesis is to produce seryl-tRNA, which is then utilized for a subsequent step in the biosynthetic pathway, biosynthetic pathway for the antibiotic valanimycin, overview Streptomyces viridifaciens ?
-
 ?
6.1.1.11 additional information proteins VlmL and SvsR, both involved in valanimycin biosynthesis, are able to catalyze a serine-dependent exchange of diphosphate into ATP and to aminoacylate total Escherichia coli tRNA with L-serine, SvsR is catalytically more efficient than VlmL, overview Streptomyces viridifaciens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.11 SerRS
-
 Streptomyces coelicolor
6.1.1.11 SerRS
-
 Streptomyces viridifaciens
6.1.1.11 SerRS2
-
 Streptomyces avermitilis
6.1.1.11 Seryl-tRNA synthetase
-
 Streptomyces coelicolor
6.1.1.11 Seryl-tRNA synthetase
-
 Streptomyces viridifaciens
6.1.1.11 SvsR
-
 Streptomyces viridifaciens
6.1.1.11 VlmL
-
 Streptomyces viridifaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.11 30
-
 ATP-diphosphate exchange assay at Streptomyces viridifaciens
6.1.1.11 35
-
 aminoacylation assay at Streptomyces viridifaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.11 7.2
-
 aminoacylation assay at Streptomyces viridifaciens
6.1.1.11 7.5
-
 ATP-diphosphate exchange assay at Streptomyces viridifaciens

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.11 ATP
-
 Streptomyces coelicolor
6.1.1.11 ATP
-
 Streptomyces avermitilis
6.1.1.11 ATP
-
 Streptomyces viridifaciens