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Literature summary extracted from

  • Maklashina, E.; Hellwig, P.; Rothery, R.A.; Kotlyar, V.; Sher, Y.; Weiner, J.H.; Cecchini, G.
    Differences in protonation of ubiquinone and menaquinone in fumarate reductase from Escherichia coli (2006), J. Biol. Chem., 281, 26655-26664.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.3.5.1 E29F mutation in subunit FrdC, dramatic decrease in enzymatic reactions with menaqunione, the succinate-ubiquinone reductase reaction remains unaffected. Elimination of the negative charge in E29 mutant enzymes results in significantly increased stabilization of both ubiquinone and menaquinone semiquinones Escherichia coli
1.3.5.1 E29F mutation in subunit FrdC, dramatic decrease in enzymatic reactions with menaqunione. Elimination of the negative charge in E29 mutant enzymes results in significantly increased stabilization of both ubiquinone and menaquinone semiquinones Escherichia coli
1.3.5.1 E29L mutation in subunit FrdC, dramatic decrease in enzymatic reactions with menaqunione, the succinate-ubiquinone reductase reaction remains unaffected. Elimination of the negative charge in E29 mutant enzymes results in significantly increased stabilization of both ubiquinone and menaquinone semiquinones Escherichia coli
1.3.5.1 E29L mutation in subunit FrdC, dramatic decrease in enzymatic reactions with menaqunione. Elimination of the negative charge in E29 mutant enzymes results in significantly increased stabilization of both ubiquinone and menaquinone semiquinones Escherichia coli
1.3.5.1 K228L mutation in subunit FrdB. Residue K228 provides a strong hydrogen bond to menaquinone and is essential for reactions with both menaquinone and ubiquinone Escherichia coli
1.3.5.1 K228L mutation in subunit FrdB. Residue K228 provides a strong hydrogen bond to menaquinone and is essential for reactions with both ubiquinone and menaquinone Escherichia coli
1.3.5.1 K228R mutation in subunit FrdB. Residue K228 provides a strong hydrogen bond to menaquinone and is essential for reactions with both menaquinone and ubiquinone Escherichia coli
1.3.5.1 K228R mutation in subunit FrdB. Residue K228 provides a strong hydrogen bond to menaquinone and is essential for reactions with both ubiquinone and menaquinone Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.1 2-n-heptyl-4-hydroxyquinoline-N-oxide
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.1 0.0018
-
 menaquinone mutant E29L, pH 7.0, 30°C Escherichia coli
1.3.5.1 0.004
-
 menaquinone wild-type, pH 7.0, 30°C Escherichia coli
1.3.5.1 0.01
-
 ubiquinone mutant E29F, pH 7.9, 30°C Escherichia coli
1.3.5.1 0.027
-
 ubiquinone mutant E29L, pH 7.9, 30°C Escherichia coli
1.3.5.1 0.07
-
 ubiquinone wild-type, pH 7.9, 30°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.1 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.1 fumarate + menaquinol
-
 Escherichia coli succinate + menaquinone
-
 r
1.3.5.1 additional information enzyme operates with both natural quinones, ubiquinone and menaquinone, at a single quinone binding site. Residue Lys228 in subunit FrdB provides a strong hydrogen bond to menaquinone and is essential for reactions with both quinone types. There is similar hydrogen bonding of the C1 carbonyl of both MQ and UQ, whereas there is different hydrogen bonding for their C4 carbonyls Escherichia coli ?
-
 ?
1.3.5.1 succinate + menaquinone
-
 Escherichia coli fumarate + menaquinol
-
 r
1.3.5.1 succinate + ubiquinone
-
 Escherichia coli fumarate + ubiquinol
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.3.5.1 quinol-fumarate reductase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.5.1 0.1
-
 succinate mutant K228R, cosubstrate ubiquinone, pH 7.9, 30°C Escherichia coli
1.3.5.1 0.3
-
 succinate mutant E49L, cosubstrate menaquinone, pH 7.9, 30°C Escherichia coli
1.3.5.1 0.3
-
 succinate mutant K228L, cosubstrate ubiquinone, pH 7.9, 30°C Escherichia coli
1.3.5.1 0.3
-
 fumarate mutant K228R, cosubstrate menaquinone, pH 7.0, 30°C Escherichia coli
1.3.5.1 0.4
-
 succinate mutant E49F, cosubstrate menaquinone, pH 7.9, 30°C Escherichia coli
1.3.5.1 2.3
-
 fumarate mutant E49F, cosubstrate menaquinone, pH 7.0, 30°C Escherichia coli
1.3.5.1 5
-
 fumarate mutant E49L, cosubstrate menaquinone, pH 7.0, 30°C Escherichia coli
1.3.5.1 15
-
 succinate wild-type, cosubstrate menaquinone, pH 7.9, 30°C Escherichia coli
1.3.5.1 20.4
-
 succinate mutant E49F, cosubstrate ubiquinone, pH 7.9, 30°C Escherichia coli
1.3.5.1 23
-
 succinate mutant E49L, cosubstrate ubiquinone, pH 7.9, 30°C Escherichia coli
1.3.5.1 24
-
 succinate wild-type, cosubstrate ubiquinone, pH 7.9, 30°C Escherichia coli
1.3.5.1 230
-
 fumarate wild-type, cosubstrate menaquinone, pH 7.0, 30°C Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.5.1 0.00003
-
 2-n-heptyl-4-hydroxyquinoline-N-oxide mutant E29L, pH 7.9, 30°C Escherichia coli
1.3.5.1 0.00005
-
 2-n-heptyl-4-hydroxyquinoline-N-oxide wild-type, pH 7.0, 30°C Escherichia coli
1.3.5.1 0.00006
-
 2-n-heptyl-4-hydroxyquinoline-N-oxide mutant E29L, pH 7.9, 30°C Escherichia coli
1.3.5.1 0.0002
-
 2-n-heptyl-4-hydroxyquinoline-N-oxide wild-type, pH 7.0, 30°C Escherichia coli