Literature summary extracted from
Winkler, A.; Hartner, F.; Kutchan, T.M.; Glieder, A.; Macheroux, P.
Biochemical evidence that berberine bridge enzyme belongs to a novel family of flavoproteins containing a bi-covalently attached FAD cofactor (2006), J. Biol. Chem., 281, 21276-21285.
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.21.3.3 |
59599 |
- |
x * 59599, calculated, including FAD cofactor |
Eschscholzia californica |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.21.3.3 |
Eschscholzia californica |
- |
expression in Pichia pastoris |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
1.21.3.3 |
glycoprotein |
three potential N-glycosylation sites, one at the N-terminus, N38, and two at the C-terminus, Asn-423 and Asn-471 |
Eschscholzia californica |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
1.21.3.3 |
culture fluid |
recombinant enzyme |
Eschscholzia californica |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.21.3.3 |
(S)-reticuline + O2 |
- |
Eschscholzia californica |
(S)-scoulerine + H2O2 |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.21.3.3 |
? |
x * 59599, calculated, including FAD cofactor |
Eschscholzia californica |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
1.21.3.3 |
8.2 |
- |
(S)-reticuline |
pH 9.0, 37°C |
Eschscholzia californica |
|
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.21.3.3 |
FAD |
covalently attached to enzyme at 8alpha position to H104 and also at 6 position to thiol group of C166. anaerobic photoirradiation leads to cleavage of the linkage to C166 yielding 6-mercaptoflavin and a peptide with C replaced by A due to breakage of the C-S bond |
Eschscholzia californica |
|