Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ha, J.Y.; Min, J.Y.; Lee, S.K.; Kim, H.S.; Kim, d.o.J.; Kim, K.H.; Lee, H.H.; Kim, H.K.; Yoon, H.J.; Suh, S.W.
    Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base (2006), J. Biol. Chem., 281, 18660-18667.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.16 expressed in Escherichia coli C41(DE3) cells Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.12.16 hanging drop vapour diffusion method using 10% (w/v) polyethylene glycol monomethyl ether 5000 Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.16 H152A His152 likely functions as the catalytic base that initiates oxidation of neutral substrates by abstracting a proton from the alpha-carbon Pseudomonas aeruginosa
1.13.12.16 H152A site-directed mutagenesis. His152 likely functions as the catalytic base that initiates oxidation of neutral substrates by abstracting a proton from the alpha-carbon Pseudomonas aeruginosa
1.13.12.16 S288A mutant enzyme forms inclusion bodies when overexpressed in Escherichia coli Pseudomonas aeruginosa
1.13.12.16 S288A site-directed mutagenesis. Mutant enzyme forms inclusion bodies when overexpressed in Escherichia coli Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.16 additional information no inhibition by valeric acid and EDTA Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.16 37000
-
 dynamic light scattering analysis Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.16 Pseudomonas aeruginosa Q9I4V0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.16 HiLoad XK 16 Superdex 200 prep-grade column gel filtration and Mono Q HR5/5 column chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.16 2-nitropropane + O2
-
 Pseudomonas aeruginosa acetone + HNO2
-
 ?
1.13.12.16 2-nitropropane + O2 anionic form Pseudomonas aeruginosa acetone + HNO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.12.16 monomer 1 * 37000, dynamic light scattering analysis Pseudomonas aeruginosa
1.13.12.16 monomer monomer 1 * 37000, dynamic light scattering analysis Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
1.13.12.16 2-nitropropane dioxygenase
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.12.16 30
-
 assay at Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.16 6.5
-
 assay at Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.12.16 FMN
-
 Pseudomonas aeruginosa