EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.3.9 | in overproducing transformants, also the N- and C- terminal domain of the enzyme were cloned and expressed separately | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.3.9 | 0.2 | - |
phosphoenolpyruvate | between 0.2 and 0.4 | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.3.9 | Mg2+ | binding of phosphoenolpyruvate requires Mg2+ | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.3.9 | Escherichia coli | P08839 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.3.9 | from overproducing transformants, also the N and C terminal domain of the enzyme | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.3.9 | phosphoenolpyruvate + protein histidine | the phosphoryl group is transferred from phosphoenolpyruvate to the enzyme and to a low molecular weight phosphocarrier protein, phosphorylation rate of the enzyme increases at presence of the phosphorylated phosphocarrier protein, phosphoryl group transfer proceeds in 4 reactions | Escherichia coli | pyruvate + protein N-pros-phosphohistidine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.3.9 | dimer | only the dimer accepts the phoshphoryl group from phosphoenolpyruvate, the monomer consists of two major domains at the N- and C- termini, the C- terminal domain forms dimers before Mg2+, phosphoenolpyruvate and the N- terminal domain bind | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.3.9 | enzyme I of the phosphotransferase system | - |
Escherichia coli |
2.7.3.9 | PEP:sugar phosphotransferase system enzyme I | - |
Escherichia coli |
2.7.3.9 | phosphoenolpyruvate-protein phosphotransferase | - |
Escherichia coli |
2.7.3.9 | phosphoenolpyruvate:protein-L-histidine N-pros-phosphotransferase | - |
Escherichia coli |
2.7.3.9 | phosphotransferase system enzyme I | - |
Escherichia coli |