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Literature summary extracted from

  • Patel, H.V.; Vyas, K.A.; Mattoo, R.L.; Southworth, M.; Perler, F.B.; Comb, D.; Roseman, S.
    Properties of the C-terminal domain of enzyme I of the Escherichia coli phosphotransferase system (2006), J. Biol. Chem., 281, 17579-17587.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.9 in overproducing transformants, also the N- and C- terminal domain of the enzyme were cloned and expressed separately Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.9 0.2
-
phosphoenolpyruvate between 0.2 and 0.4 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.3.9 Mg2+ binding of phosphoenolpyruvate requires Mg2+ Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.9 Escherichia coli P08839
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.9 from overproducing transformants, also the N and C terminal domain of the enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.9 phosphoenolpyruvate + protein histidine the phosphoryl group is transferred from phosphoenolpyruvate to the enzyme and to a low molecular weight phosphocarrier protein, phosphorylation rate of the enzyme increases at presence of the phosphorylated phosphocarrier protein, phosphoryl group transfer proceeds in 4 reactions Escherichia coli pyruvate + protein N-pros-phosphohistidine
-
?

Subunits

EC Number Subunits Comment Organism
2.7.3.9 dimer only the dimer accepts the phoshphoryl group from phosphoenolpyruvate, the monomer consists of two major domains at the N- and C- termini, the C- terminal domain forms dimers before Mg2+, phosphoenolpyruvate and the N- terminal domain bind Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.3.9 enzyme I of the phosphotransferase system
-
Escherichia coli
2.7.3.9 PEP:sugar phosphotransferase system enzyme I
-
Escherichia coli
2.7.3.9 phosphoenolpyruvate-protein phosphotransferase
-
Escherichia coli
2.7.3.9 phosphoenolpyruvate:protein-L-histidine N-pros-phosphotransferase
-
Escherichia coli
2.7.3.9 phosphotransferase system enzyme I
-
Escherichia coli