EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.179 | expression of wild-type and mutant His-tagged FabF in Escherichia coli strain BL21(DE3) | Streptococcus pneumoniae |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.3.1.179 | purified recombinant mutant H303A from 20% polyethylene glycol 3350, 0.2 M potassium acetate, X-ray diffraction structure determination and analysis | Streptococcus pneumoniae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.179 | C164A | site-directed mutagenesis, inactive mutant | Streptococcus pneumoniae |
2.3.1.179 | C164A/H337A | site-directed mutagenesis, inactive mutant | Streptococcus pneumoniae |
2.3.1.179 | C164A/K332A | site-directed mutagenesis, inactive mutant | Streptococcus pneumoniae |
2.3.1.179 | E346A | site-directed mutagenesis, the mutant shows similar activity compared to the wild-type enzyme | Streptococcus pneumoniae |
2.3.1.179 | E396A | site-directed mutagenesis, the mutant shows no condensation activity but retains about 50% of wild-type transacylation activity with acyl-ACP and ACP, and 40% of wild-type decarboxylation activity | Streptococcus pneumoniae |
2.3.1.179 | H303A | site-directed mutagenesis, the mutant shows 74% reduced condensation activity, 40% reduced transacylation activity, and 5fold increased decarboxylation activity, compared to the wild-type enzyme | Streptococcus pneumoniae |
2.3.1.179 | H337A | site-directed mutagenesis, inactive mutant | Streptococcus pneumoniae |
2.3.1.179 | K332A | site-directed mutagenesis, the mutant shows no condensation activity but retains about 30% of wild-type transacylation activity with acyl-ACP and ACP, and 10% of wild-type decarboxylation activity | Streptococcus pneumoniae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.179 | myristoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Streptococcus pneumoniae | - |
? + CO2 + [acyl-carrier protein] | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.179 | Streptococcus pneumoniae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.179 | recombinant wild-type and mutant His-tagged FabF from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Streptococcus pneumoniae |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.3.1.179 | a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a (Z)-3-oxooctadec-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | elongation condensing enzyme, catalytic mechanism involving Cys134, His337, and His303, forming the catalytic triad, as well as Phe396, and a water molecule bound to the active site, analysis of residues involved in the different reaction steps, overview | Streptococcus pneumoniae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.179 | additional information | analysis of interaction between FabF and the acyl-carrier protein | Streptococcus pneumoniae | ? | - |
? | |
2.3.1.179 | myristoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Streptococcus pneumoniae | ? + CO2 + [acyl-carrier protein] | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.179 | beta-ketoacyl-ACP synthase II | - |
Streptococcus pneumoniae |
2.3.1.179 | FabF | - |
Streptococcus pneumoniae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.179 | 37 | - |
assay at | Streptococcus pneumoniae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.179 | 6.8 | 7 | assay at | Streptococcus pneumoniae |