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Literature summary extracted from

  • Sellick, C.A.; Reece, R.J.
    Contribution of amino acid side chains to sugar binding specificity in a galactokinase, Gal1p, and a transcriptional inducer, Gal3p (2006), J. Biol. Chem., 281, 17150-17155.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.6 D62A abolishes all detectable galactokinase activity but retains the ability to use D-glucose as a substrate Saccharomyces cerevisiae
2.7.1.6 D62A/Y274A shows no sugar kinase activity Saccharomyces cerevisiae
2.7.1.6 D62C shows no sugar kinase activity Saccharomyces cerevisiae
2.7.1.6 D62E shows no detectable sugar kinase activity Saccharomyces cerevisiae
2.7.1.6 D62F abolishes all detectable galactokinase activity but retains the ability to use D-glucose as a substrate Saccharomyces cerevisiae
2.7.1.6 D62G shows no sugar kinase activity Saccharomyces cerevisiae
2.7.1.6 D62H abolishes all detectable galactokinase activity but retains the ability to use D-glucose as a substrate Saccharomyces cerevisiae
2.7.1.6 D62I shows no sugar kinase activity Saccharomyces cerevisiae
2.7.1.6 D62K shows no detectable sugar kinase activity Saccharomyces cerevisiae
2.7.1.6 D62L abolishes all detectable galactokinase activity but retains the ability to use D-glucose as a substrate Saccharomyces cerevisiae
2.7.1.6 D62M shows no sugar kinase activity Saccharomyces cerevisiae
2.7.1.6 D62N shows no detectable sugar kinase activity Saccharomyces cerevisiae
2.7.1.6 D62P shows no sugar kinase activity Saccharomyces cerevisiae
2.7.1.6 D62Q shows no sugar kinase activity Saccharomyces cerevisiae
2.7.1.6 D62R shows no sugar kinase activity Saccharomyces cerevisiae
2.7.1.6 D62S shows no sugar kinase activity Saccharomyces cerevisiae
2.7.1.6 D62T shows no sugar kinase activity Saccharomyces cerevisiae
2.7.1.6 D62V shows no sugar kinase activity Saccharomyces cerevisiae
2.7.1.6 D62W shows no sugar kinase activity Saccharomyces cerevisiae
2.7.1.6 D62Y shows no sugar kinase activity Saccharomyces cerevisiae
2.7.1.6 R53A/D217A shows no sugar kinase activity Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.6 0.038
-
 D-glucose mutant enzyme D62F, at 30°C Saccharomyces cerevisiae
2.7.1.6 0.046
-
 ATP in the presence of 2-deoxy-D-galactose as a substrate, at 30°C, wild type enzyme Saccharomyces cerevisiae
2.7.1.6 0.057
-
 ATP in the presence of D-galactose as a substrate, wild type enzyme Saccharomyces cerevisiae
2.7.1.6 0.107
-
 D-glucose mutant enzyme D62A, at 30°C Saccharomyces cerevisiae
2.7.1.6 0.137
-
 D-glucose mutant enzyme D62L, at 30°C Saccharomyces cerevisiae
2.7.1.6 0.148
-
 D-glucose mutant enzyme D62H, at 30°C Saccharomyces cerevisiae
2.7.1.6 0.225
-
 ATP in the presence of D-glucose as a substrate, at 30°C, wild type enzyme Saccharomyces cerevisiae
2.7.1.6 0.225
-
 D-glucose wild type enzyme, at 30°C Saccharomyces cerevisiae
2.7.1.6 0.271
-
 ATP mutant enzyme D62L, at 30°C Saccharomyces cerevisiae
2.7.1.6 0.296
-
 ATP mutant enzyme D62F, at 30°C Saccharomyces cerevisiae
2.7.1.6 0.34
-
 ATP mutant enzyme D62A, at 30°C Saccharomyces cerevisiae
2.7.1.6 0.371
-
 ATP mutant enzyme D62H, at 30°C Saccharomyces cerevisiae
2.7.1.6 0.539
-
 D-galactose wild type enzyme, at 30°C Saccharomyces cerevisiae
2.7.1.6 29.21
-
 2-deoxy-D-galactose wild type enzyme, at 30°C Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.6 Saccharomyces cerevisiae
-
-
-
2.7.1.6 Saccharomyces cerevisiae mc2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.6 Ni2+-nitrilotriacetic acid-agarose column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.6 ATP + 2-deoxy-D-galactose
-
 Saccharomyces cerevisiae ADP + 2-deoxy-alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + 2-deoxy-D-galactose
-
 Saccharomyces cerevisiae mc2 ADP + 2-deoxy-alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + D-galactose
-
 Saccharomyces cerevisiae ADP + alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + D-galactose
-
 Saccharomyces cerevisiae mc2 ADP + alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + D-glucose
-
 Saccharomyces cerevisiae ADP + alpha-D-glucose 1-phosphate
-
 ?
2.7.1.6 ATP + D-glucose
-
 Saccharomyces cerevisiae mc2 ADP + alpha-D-glucose 1-phosphate
-
 ?
2.7.1.6 additional information L-arabinose, D-fucose, L-glucose, and D-talose are no substrates Saccharomyces cerevisiae ?
-
 ?
2.7.1.6 additional information L-arabinose, D-fucose, L-glucose, and D-talose are no substrates Saccharomyces cerevisiae mc2 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.6 Gal1p
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.6 0.007
-
 D-glucose mutant enzyme D62F, at 30°C Saccharomyces cerevisiae
2.7.1.6 0.019
-
 D-glucose mutant enzyme D62A, at 30°C Saccharomyces cerevisiae
2.7.1.6 0.034
-
 D-glucose wild type enzyme, at 30°C Saccharomyces cerevisiae
2.7.1.6 0.037
-
 D-glucose mutant enzyme D62L, at 30°C Saccharomyces cerevisiae
2.7.1.6 0.067
-
 D-glucose mutant enzyme D62H, at 30°C Saccharomyces cerevisiae
2.7.1.6 3.13
-
 2-deoxy-D-galactose wild type enzyme, at 30°C Saccharomyces cerevisiae
2.7.1.6 4.01
-
 2-deoxy-D-galactose wild type enzyme, at 30°C Saccharomyces cerevisiae
2.7.1.6 22.3
-
 D-galactose wild type enzyme, at 30°C Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.6 ATP
-
 Saccharomyces cerevisiae