EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.11.16 | expression of GRK6 in both Spodoptera frugiperda Sf9 and Trichoplusia ni High5 insect cells as a soluble, palmitoylation-deficient mutant in which three potential palmitoylation sites, located at Cys561, Cys562, and Cys565, are converted to Ser | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.11.16 | C561S/C562S/C565S | site-directed mutagenesis, mutation of palmitoylation sites, the mutant protein retains its ability to phosphorylate rhodopsin, albeit with a 5fold higher Km and 2fold lower Vmax compared with those of wild-type GRK6 | Homo sapiens |
2.7.11.16 | F527D | site-directed mutagenesis, mutant structure in comparison to the wild-type enzyme | Homo sapiens |
2.7.11.16 | I165E | site-directed mutagenesis, mutant structure in comparison to the wild-type enzyme | Homo sapiens |
2.7.11.16 | I165E/F527D | site-directed mutagenesis, mutant structure in comparison to the wild-type enzyme | Homo sapiens |
2.7.11.16 | I39E | site-directed mutagenesis, mutant structure in comparison to the wild-type enzyme | Homo sapiens |
2.7.11.16 | I39E/I165E | site-directed mutagenesis, mutant structure in comparison to the wild-type enzyme | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.11.16 | cell membrane | association of GRK6 with the cell membrane is mediated in part by the palmitoylation of cysteine residues that lie in a C-terminal region | Homo sapiens | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.16 | Mg2+ | - |
Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.11.16 | 66050 | - |
2 * 66050, recombinant acetylated, full-length, palmitoylation-deficient GRK6, mass spectrometry | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.11.16 | ATP + rhodopsin | Homo sapiens | - |
ADP + phosphorylated rhodopsin | - |
? | |
2.7.11.16 | additional information | Homo sapiens | GRK6 is a key regulator of dopaminergic signaling and lymphocyte chemotaxis | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.11.16 | Homo sapiens | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.7.11.16 | lipoprotein | GRK6 is palmitoylated at Cys561, Cys562, and Cys565 | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.11.16 | ATP + rhodopsin | - |
Homo sapiens | ADP + phosphorylated rhodopsin | - |
? | |
2.7.11.16 | additional information | GRK6 is a key regulator of dopaminergic signaling and lymphocyte chemotaxis | Homo sapiens | ? | - |
? | |
2.7.11.16 | additional information | GRK6 structure-function relationship determination and analysis | Homo sapiens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.11.16 | dimer | 2 * 66050, recombinant acetylated, full-length, palmitoylation-deficient GRK6, mass spectrometry | Homo sapiens |
2.7.11.16 | More | GRK6 structure-function relationship determination and analysis, the structure involves the RH-kinase domain core, and small and large kinase lobes, comparison to the structure of GRK2, EC 2.7.11.15, overview | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.11.16 | G protein-coupled receptor kinase-6 | - |
Homo sapiens |
2.7.11.16 | GRK-6 | - |
Homo sapiens |
2.7.11.16 | More | GRK6 is a member of the GRK4 subfamily of GRKs | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.16 | ATP | - |
Homo sapiens |