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Literature summary extracted from

  • Lodowski, D.T.; Tesmer, V.M.; Benovic, J.L.; Tesmer, J.J.
    The structure of G protein-coupled receptor kinase (GRK)-6 defines a second lineage of GRKs (2006), J. Biol. Chem., 281, 16785-16793.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.16 expression of GRK6 in both Spodoptera frugiperda Sf9 and Trichoplusia ni High5 insect cells as a soluble, palmitoylation-deficient mutant in which three potential palmitoylation sites, located at Cys561, Cys562, and Cys565, are converted to Ser Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.16 C561S/C562S/C565S site-directed mutagenesis, mutation of palmitoylation sites, the mutant protein retains its ability to phosphorylate rhodopsin, albeit with a 5fold higher Km and 2fold lower Vmax compared with those of wild-type GRK6 Homo sapiens
2.7.11.16 F527D site-directed mutagenesis, mutant structure in comparison to the wild-type enzyme Homo sapiens
2.7.11.16 I165E site-directed mutagenesis, mutant structure in comparison to the wild-type enzyme Homo sapiens
2.7.11.16 I165E/F527D site-directed mutagenesis, mutant structure in comparison to the wild-type enzyme Homo sapiens
2.7.11.16 I39E site-directed mutagenesis, mutant structure in comparison to the wild-type enzyme Homo sapiens
2.7.11.16 I39E/I165E site-directed mutagenesis, mutant structure in comparison to the wild-type enzyme Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.16 cell membrane association of GRK6 with the cell membrane is mediated in part by the palmitoylation of cysteine residues that lie in a C-terminal region Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.16 Mg2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.16 66050
-
 2 * 66050, recombinant acetylated, full-length, palmitoylation-deficient GRK6, mass spectrometry Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.16 ATP + rhodopsin Homo sapiens
-
 ADP + phosphorylated rhodopsin
-
 ?
2.7.11.16 additional information Homo sapiens GRK6 is a key regulator of dopaminergic signaling and lymphocyte chemotaxis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.16 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.16 lipoprotein GRK6 is palmitoylated at Cys561, Cys562, and Cys565 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.16 ATP + rhodopsin
-
 Homo sapiens ADP + phosphorylated rhodopsin
-
 ?
2.7.11.16 additional information GRK6 is a key regulator of dopaminergic signaling and lymphocyte chemotaxis Homo sapiens ?
-
 ?
2.7.11.16 additional information GRK6 structure-function relationship determination and analysis Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.16 dimer 2 * 66050, recombinant acetylated, full-length, palmitoylation-deficient GRK6, mass spectrometry Homo sapiens
2.7.11.16 More GRK6 structure-function relationship determination and analysis, the structure involves the RH-kinase domain core, and small and large kinase lobes, comparison to the structure of GRK2, EC 2.7.11.15, overview Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.11.16 G protein-coupled receptor kinase-6
-
 Homo sapiens
2.7.11.16 GRK-6
-
 Homo sapiens
2.7.11.16 More GRK6 is a member of the GRK4 subfamily of GRKs Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.16 ATP
-
 Homo sapiens