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Literature summary extracted from

  • Wijma, H.J.; Jeuken, L.J.; Verbeet, M.P.; Armstrong, F.A.; Canters, G.W.
    A random-sequential mechanism for nitrite binding and active site reduction in copper-containing nitrite reductase (2006), J. Biol. Chem., 281, 16340-16346.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.7.2.1 M150T mutant enzyme has a type-1 site with a 125-mV higher midpoint potential and a 0.3-eV higher reorganization energy leading to an about 50-fold slower intramolecular electrontransfer to the type-2 site Alcaligenes faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.1 additional information
-
 additional information
-
 Alcaligenes faecalis

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.1 Alcaligenes faecalis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.1 nitrite + reduced benzyl viologen random sequential mechanism Alcaligenes faecalis NO + oxidized benzyl viologen
-
 ?
1.7.2.1 nitrite + reduced methyl viologen random sequential mechanism Alcaligenes faecalis NO + oxidized methyl viologen
-
 ?
1.7.2.1 nitrite + reduced pseudoazurin random sequential mechanism Alcaligenes faecalis NO + oxidized pseudoazurin
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.1 5.6
-
 reaction with 0.5 mM nitrite and pseudoazurin as electron donor Alcaligenes faecalis
1.7.2.1 6
-
 reaction with 5 mM nitrite and pseudoazurin as electron donor Alcaligenes faecalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.2.1 4.9 6.2 pH 4.9: about 55% of maximal ativity, pH 6.2: about 65% of maximal activity, reaction with 0.5 mM nitrite and pseudoazurin as electron donor Alcaligenes faecalis
1.7.2.1 5.2 6.5 pH 5.2: about 60% of maximal activity, pH 6.5: about 55% of maximal activity, reaction with 5 mM nitrite and pseudoazurin as electron donor Alcaligenes faecalis