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Literature summary extracted from

  • Viswanathan, K.; Tomiya, N.; Park, J.; Singh, S.; Lee, Y.C.; Palter, K.; Betenbaugh, M.J.
    Expression of a functional Drosophila melanogaster CMP-sialic acid synthetase. Differential localization of the Drosophila and human enzymes (2006), J. Biol. Chem., 281, 15929-15940.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.43 DmCSAS is cloned into the baculovirus vector pBlueBac4.5 (pBlueBac-DmCSAS4) which is used to transfect LEC29.Lec32 cells Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.43 additional information in contrast to its mammalian counterparts DmCSAS protein begins with an N-terminal sequence rich in hydrophobic amino acids characteristic of a signal/anchoring sequence Drosophila melanogaster
2.7.7.43 additional information multiple alignment among the Drosophila melanogaster, human, mouse, Escherichia coli and Neisseria meningitidis CSAS enzymes shows that all proteins are homologous over the length of the catalytic domain Drosophila melanogaster
2.7.7.43 additional information replacement of the N-terminal leader sequence of DmCSAS with the human CSAS N-terminal sequence results in the redirection of the chimeric CSAS protein to the nucleus but with concomitant loss of activity Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.43 Golgi apparatus unlike all vertebrate CSAS that localize to the nucleus, the DmCSAS is localized to the Golgi compartment Drosophila melanogaster 5794
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2.7.7.43 additional information replacement of the N-terminal leader sequence of DmCSAS with the human CSAS N-terminal sequence results in the redirection of the chimeric CSAS protein to the nucleus but with concomitant loss of activity Drosophila melanogaster
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.43 Mg2+ assay with 20 mM Mg2+ Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.43 27500
-
 predicted from cDNA Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.43 Drosophila melanogaster
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.43 head DmCSAS gene is greatly enriched in adult head, suggesting a possible role in the central nervous system Drosophila melanogaster
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2.7.7.43 additional information DmCSAS gene is developmentally regulated, with expression first appearing at 12-24 h of embryogenesis, low expression through larval and pubal stages Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.43 N-acetylneuraminic acid + CTP DmCSAS is shown to be functional both in vitro and in vivo Drosophila melanogaster CMP-Neu5Ac + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.43 CMP-sialic acid synthetase
-
 Drosophila melanogaster
2.7.7.43 DmCSAS
-
 Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.43 37
-
 assay at Drosophila melanogaster