EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.43 | DmCSAS is cloned into the baculovirus vector pBlueBac4.5 (pBlueBac-DmCSAS4) which is used to transfect LEC29.Lec32 cells | Drosophila melanogaster |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.7.43 | additional information | in contrast to its mammalian counterparts DmCSAS protein begins with an N-terminal sequence rich in hydrophobic amino acids characteristic of a signal/anchoring sequence | Drosophila melanogaster |
2.7.7.43 | additional information | multiple alignment among the Drosophila melanogaster, human, mouse, Escherichia coli and Neisseria meningitidis CSAS enzymes shows that all proteins are homologous over the length of the catalytic domain | Drosophila melanogaster |
2.7.7.43 | additional information | replacement of the N-terminal leader sequence of DmCSAS with the human CSAS N-terminal sequence results in the redirection of the chimeric CSAS protein to the nucleus but with concomitant loss of activity | Drosophila melanogaster |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.7.43 | Golgi apparatus | unlike all vertebrate CSAS that localize to the nucleus, the DmCSAS is localized to the Golgi compartment | Drosophila melanogaster | 5794 | - |
2.7.7.43 | additional information | replacement of the N-terminal leader sequence of DmCSAS with the human CSAS N-terminal sequence results in the redirection of the chimeric CSAS protein to the nucleus but with concomitant loss of activity | Drosophila melanogaster | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.43 | Mg2+ | assay with 20 mM Mg2+ | Drosophila melanogaster |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.7.43 | 27500 | - |
predicted from cDNA | Drosophila melanogaster |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.43 | Drosophila melanogaster | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.7.43 | head | DmCSAS gene is greatly enriched in adult head, suggesting a possible role in the central nervous system | Drosophila melanogaster | - |
2.7.7.43 | additional information | DmCSAS gene is developmentally regulated, with expression first appearing at 12-24 h of embryogenesis, low expression through larval and pubal stages | Drosophila melanogaster | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.43 | N-acetylneuraminic acid + CTP | DmCSAS is shown to be functional both in vitro and in vivo | Drosophila melanogaster | CMP-Neu5Ac + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.43 | CMP-sialic acid synthetase | - |
Drosophila melanogaster |
2.7.7.43 | DmCSAS | - |
Drosophila melanogaster |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.43 | 37 | - |
assay at | Drosophila melanogaster |