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Literature summary extracted from

  • Boniface, A.; Bouhss, A.; Mengin-Lecreulx, D.; Blanot, D.
    The MurE synthetase from Thermotoga maritima is endowed with an unusual D-lysine adding activity (2006), J. Biol. Chem., 281, 15680-15686.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.13 expressed in Escherichia coli strains BL21(DE3)/pLysS and DH5alpha Thermotoga maritima
6.3.2.37 expressed as C-terminal His6-tagged enzyme in Escherichia coli DH5alpha cells Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.37 UDP-N-acetylmuramoyl-L-Ala-D-Glu strong inhibition by excess UDP-N-acetylmuramoyl-L-Ala-D-Glu is observed Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.13 0.45
-
 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate at 68°C Thermotoga maritima
6.3.2.13 1.7
-
 D-Lysine at 68°C Thermotoga maritima
6.3.2.13 2.8
-
 L-lysine at 68°C Thermotoga maritima
6.3.2.13 3.6
-
 ATP at 68°C Thermotoga maritima
6.3.2.13 4.8
-
 meso-diaminopimelic acid at 68°C Thermotoga maritima
6.3.2.13 27
-
 L-ornithine at 68°C Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.13 Mg2+ 15 mM for optimal activity Thermotoga maritima
6.3.2.37 Mg2+ the optimum Mg2+ concentration for L-lysine adding activity is 15 mM Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.13 56000
-
 SDS-PAGE Thermotoga maritima
6.3.2.37 56000
-
 SDS-PAGE Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.13 Thermotoga maritima
-
-
-
6.3.2.37 Thermotoga maritima
-
-
-
6.3.2.37 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.13 Ni2+-nitrilotriacetate-agarose chromatography Thermotoga maritima
6.3.2.37 Ni-NTA agarose column chromatography, gel filtration Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.13 ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + D-lysine
-
 Thermotoga maritima ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-D-lysine
-
 ?
6.3.2.13 ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + D-lysine
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-D-lysine
-
 ?
6.3.2.13 ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-lysine
-
 Thermotoga maritima ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysine
-
 ?
6.3.2.13 ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-lysine
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysine
-
 ?
6.3.2.13 ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-ornithine
-
 Thermotoga maritima ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-ornithine
-
 ?
6.3.2.13 ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-ornithine
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-ornithine
-
 ?
6.3.2.13 ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-diaminopimelic acid
-
 Thermotoga maritima ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid
-
 ?
6.3.2.13 ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-diaminopimelic acid
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid
-
 ?
6.3.2.37 UDP-N-acetylmuramoyl-L-Ala-D-Glu + D-Lys + ATP D-lysine is added to UDP-N-acetylmuramoyl-L-Ala-D-Glu with 25% efficiency relative to L-lysine Thermotoga maritima UDP-N-acetylmuramoyl-L-Ala-D-Glu-D-Lys + ADP + phosphate
-
 ?
6.3.2.37 UDP-N-acetylmuramoyl-L-Ala-D-Glu + D-Lys + ATP D-lysine is added to UDP-N-acetylmuramoyl-L-Ala-D-Glu with 25% efficiency relative to L-lysine Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 UDP-N-acetylmuramoyl-L-Ala-D-Glu-D-Lys + ADP + phosphate
-
 ?
6.3.2.37 UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys + ATP
-
 Thermotoga maritima UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + ADP + phosphate
-
 ?
6.3.2.37 UDP-N-acetylmuramoyl-L-Ala-D-Glu + meso-diaminopimelic acid + ATP meso-diaminopimelic acid is added to UDP-N-acetylmuramoyl-L-Ala-D-Glu with 10% efficiency relative to L-lysine Thermotoga maritima UDP-N-acetylmuramoyl-L-Ala-D-Glu-meso-diaminopimelic acid + ADP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.13 MurE synthetase
-
 Thermotoga maritima
6.3.2.37 MurE
-
 Thermotoga maritima
6.3.2.37 MurE synthetase
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.13 68
-
-
 Thermotoga maritima
6.3.2.37 68
-
 temperature optimum for L-lysine adding activity. At 37°C, the activity is about 25% of the optimal value Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.3.2.13 37
-
 the enzyme has 25% of its maximum activity at 37°C Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.37 9.4
-
 for L-lysine adding activity Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.2.13 9.4
-
-
 Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.37 ATP
-
 Thermotoga maritima