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Literature summary extracted from

  • Man, W.C.; Miyazaki, M.; Chu, K.; Ntambi, J.M.
    Membrane topology of mouse stearoyl-CoA desaturase 1 (2006), J. Biol. Chem., 281, 1251-1260.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.1 expressed in HeLa cells, tagged with myc epitope at the C terminus or HA epitope Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.19.1 C222S with Myc epitope tagged to the C terminus, expressed in HeLa cells, actitivity comparable to that of the wild-type Mus musculus
1.14.19.1 C233S with Myc epitope tagged to the C terminus, expressed in HeLa cells, actitivity comparable to that of the wild-type Mus musculus
1.14.19.1 C322S with Myc epitope tagged to the C terminus, expressed in HeLa cells, actitivity comparable to that of the wild-type Mus musculus
1.14.19.1 C92S with Myc epitope tagged to the C terminus, expressed in HeLa cells, actitivity comparable to that of the wild-type Mus musculus
1.14.19.1 C92S/C97S/C222S/C322S with Myc epitope tagged to the C terminus, expressed in HeLa cells, actitivity 5fold increased compared with cells transfected with the empty vector, clear ER pattern Mus musculus
1.14.19.1 C97S with Myc epitope tagged to the C terminus, expressed in HeLa cells, actitivity comparable to that of the wild-type Mus musculus
1.14.19.1 additional information activities of enzymes with HA epitope tagged to the N terminus and Myc epitope tagged to the C terminus expressed in HeLa cells are 23fold and 39fold higher than in cells transfected with an empty vector, acitivities of enzymes with HA epitope after amino acid 93 and 138 additionally Myc epitope tagged to the C terminus are 5.5fold and 5.9fold higher than in cells transfected with an empty vector, respectively Mus musculus
1.14.19.1 additional information Myc epitope at the C terminus and additional HA epitope at predicted loops, after amino acid 93, 138 or 241 Mus musculus
1.14.19.1 additional information no activity when HA epitope is inserted after amino acid 241 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.1 microsome from HeLa cells expressing stearoyl-CoA 9-desaturase Mus musculus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.19.1 41000
-
 Myc or HA epitope tagged proteins expressed in HeLa cells, immunoblot using anti-Myc and ant-HA antibodies Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.1 Mus musculus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.1 stearoyl-CoA + NADH + O2
-
 Mus musculus oleoyl-CoA + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.1 More N and C termini are oriented toward the cytosol, loop between the first and second transmembrane domain is oriented toward the ER lumen, the loop between the second and third transmembrane domain is oriented toward the cytosol, the loop between the third and fourth transmembrane domain is oriented toward the ER lumen, indirect immunofluorescence of transiently transfected HeLa cells Mus musculus

Synonyms

EC Number Synonyms Comment Organism
1.14.19.1 SCD1
-
 Mus musculus