EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.3.5.1 | hanging drop vapor diffusion method, x-ray structure of mutant E49Q | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.5.1 | E49A | decrease in catalytic efficiency of both fumarate reduction and succinate oxidation | Escherichia coli |
1.3.5.1 | E49Q | decrease in catalytic efficiency of both fumarate reduction and succinate oxidation | Escherichia coli |
1.3.5.1 | additional information | investigation on the role of the amino acid side chain in enzymes with Glu/Gln/Ala substitutions at fumarate reductase FrdA Glu49 and succinate dehydrogenase SdhA, EC 1.5.3.1, Gln50. The mutant enzymes with Ala substitutions in either Frd or Sdh remain functionally similar to their wild type counterparts. There are, however, dramatic changes in the catalytic properties when Glu and Gln are exchanged for each other in Frd and Sdh. Both enzymes are more efficient succinate oxidases when Gln is in the target position and a better fumarate reductase when Glu is present. Structural and catalytic analyses of the FrdA E49Q and SdhA Q50E mutants suggest that coulombic effects and the electronic state of the FAD are critical in dictating the preferred directionality of the succinate/fumarate interconversions | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.5.1 | malonate | - |
Escherichia coli | |
1.3.5.1 | oxaloacetate | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.5.1 | 0.02 | - |
fumarate | wild-type, pH 7.0, 30°C | Escherichia coli | |
1.3.5.1 | 0.03 | - |
fumarate | mutant E49A, pH 7.0, 30°C | Escherichia coli | |
1.3.5.1 | 0.11 | - |
succinate | mutant E49A, pH 7.0, 30°C | Escherichia coli | |
1.3.5.1 | 0.22 | - |
succinate | mutant E49A, pH 7.0, 30°C | Escherichia coli | |
1.3.5.1 | 0.55 | - |
succinate | wild-type, pH 7.0, 30°C | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.5.1 | Escherichia coli | P00363 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.5.1 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.5.1 | fumarate + menaquinol | - |
Escherichia coli | succinate + menaquinone | - |
r | |
1.3.5.1 | succinate + menaquinone | - |
Escherichia coli | fumarate + menaquinol | - |
r |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.5.1 | 1 | - |
fumarate | mutant E49A, pH 7.0, 30°C | Escherichia coli | |
1.3.5.1 | 2.4 | - |
succinate | mutant E49A, pH 7.0, 30°C | Escherichia coli | |
1.3.5.1 | 4 | - |
succinate | mutant E49A, pH 7.0, 30°C | Escherichia coli | |
1.3.5.1 | 30 | - |
succinate | wild-type, pH 7.0, 30°C | Escherichia coli | |
1.3.5.1 | 32 | - |
fumarate | mutant E49A, pH 7.0, 30°C | Escherichia coli | |
1.3.5.1 | 250 | - |
fumarate | wild-type, pH 7.0, 30°C | Escherichia coli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.5.1 | 0.00006 | - |
oxaloacetate | mutant E49A, pH 7.0, 30°C | Escherichia coli | |
1.3.5.1 | 0.0001 | - |
oxaloacetate | mutant E49A, pH 7.0, 30°C | Escherichia coli | |
1.3.5.1 | 0.0003 | - |
oxaloacetate | wild-type, pH 7.0, 30°C | Escherichia coli | |
1.3.5.1 | 0.005 | - |
malonate | mutant E49A, pH 7.0, 30°C | Escherichia coli | |
1.3.5.1 | 0.01 | - |
malonate | mutant E49A, pH 7.0, 30°C | Escherichia coli | |
1.3.5.1 | 0.025 | - |
malonate | wild-type, pH 7.0, 30°C | Escherichia coli |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.5.1 | 11 | - |
succinate | mutant E49A, pH 7.0, 30°C | Escherichia coli | |
1.3.5.1 | 36 | - |
succinate | mutant E49A, pH 7.0, 30°C | Escherichia coli | |
1.3.5.1 | 54 | - |
succinate | wild-type, pH 7.0, 30°C | Escherichia coli | |
1.3.5.1 | 97 | - |
fumarate | mutant E49A, pH 7.0, 30°C | Escherichia coli | |
1.3.5.1 | 232 | - |
fumarate | wild-type, pH 7.0, 30°C | Escherichia coli |