EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.21 | expression of wild-type and mutant isozymes M-CPT I in Pichia pastoris strain GS115 | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.21 | A305C | site-directed mutagenesis, the revertant mutant shows about wild-type activity | Homo sapiens |
2.3.1.21 | C305A | site-directed mutagenesis, inactive mutant | Homo sapiens |
2.3.1.21 | C448A | site-directed mutagenesis, the mutant shows unaltered catalytic activity and sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
2.3.1.21 | C504A | site-directed mutagenesis, the mutant shows unaltered catalytic activity and sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
2.3.1.21 | C526A | site-directed mutagenesis, the mutant shows unaltered catalytic activity but increased sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
2.3.1.21 | C548S | site-directed mutagenesis, the mutant shows unaltered catalytic activity and sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
2.3.1.21 | C562A | site-directed mutagenesis, the mutant shows unaltered catalytic activity and sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
2.3.1.21 | C586A | site-directed mutagenesis, the mutant shows unaltered catalytic activity but reduced sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
2.3.1.21 | C608A | site-directed mutagenesis, the mutant shows unaltered catalytic activity and sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
2.3.1.21 | C659A | site-directed mutagenesis, the mutant shows unaltered catalytic activity but increased sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.21 | malonyl-CoA | IC50: 0.0005 mM for the recombinant wild-type enzyme, IC50: 0.00052 mM for the recombinant revertant A305C, IC50 values of recombinant mutant isozymes M-CPT I, overview | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.21 | 0.066 | - |
palmitoyl-CoA | pH 8.0, 37°C, recombinant revertant A305C | Homo sapiens | |
2.3.1.21 | 0.073 | - |
palmitoyl-CoA | pH 8.0, 37°C, recombinant wild-type enzyme | Homo sapiens | |
2.3.1.21 | 0.824 | - |
L-carnitine | pH 8.0, 37°C, recombinant wild-type enzyme | Homo sapiens | |
2.3.1.21 | 0.92 | - |
L-carnitine | pH 8.0, 37°C, recombinant revertant A305C | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.1.21 | mitochondrion | - |
Homo sapiens | 5739 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.21 | palmitoyl-CoA + L-carnitine | Homo sapiens | CPT I catalyzes the conversion of long-chain fatty acyl-CoAs to acyl carnitines in the presence of L-carnitine, a rate-limiting step in the transport of long-chain fatty acids from the cytoplasm to the mitochondrial matrix | CoA + L-palmitoylcarnitine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.21 | Homo sapiens | - |
isozyme M-CPT I | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.3.1.21 | palmitoyl-CoA + L-carnitine = CoA + L-palmitoylcarnitine | the catalytic triad is composed of Cys305, His473, and Asp454, with Cys305 serving as a probable nucleophile, thus acting as a site for covalent attachment of the acyl molecule and formation of a stable acyl-enzyme intermediate, mechanism | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.1.21 | heart | isozyme M-CPT I | Homo sapiens | - |
2.3.1.21 | muscle | isozyme M-CPT I | Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.21 | additional information | - |
activity of mutant enzymes | Homo sapiens |
2.3.1.21 | 0.0028 | - |
recombinant revertant A305C | Homo sapiens |
2.3.1.21 | 0.0029 | - |
recombinant wild-type enzyme | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.21 | palmitoyl-CoA + L-carnitine | - |
Homo sapiens | CoA + L-palmitoylcarnitine | - |
? | |
2.3.1.21 | palmitoyl-CoA + L-carnitine | CPT I catalyzes the conversion of long-chain fatty acyl-CoAs to acyl carnitines in the presence of L-carnitine, a rate-limiting step in the transport of long-chain fatty acids from the cytoplasm to the mitochondrial matrix | Homo sapiens | CoA + L-palmitoylcarnitine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.21 | muscle carnitine palmitoyltransferase I | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.21 | 30 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.21 | 7.5 | - |
assay at | Homo sapiens |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.3.1.21 | 0.0005 | - |
IC50: 0.0005 mM for the recombinant wild-type enzyme | Homo sapiens | malonyl-CoA | |
2.3.1.21 | 0.00052 | - |
IC50: 0.00052 mM for the recombinant revertant A305C, IC50 values of recombinant mutant isozymes M-CPT I overview | Homo sapiens | malonyl-CoA |