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Literature summary extracted from

  • Forouhar, F.; Lee, I.S.; Vujcic, J.; Vujcic, S.; Shen, J.; Vorobiev, S.M.; Xiao, R.; Acton, T.B.; Montelione, G.T.; Porter, C.W.; Tong, L.
    Structural and functional evidence for Bacillus subtilis PaiA as a novel N1-spermidine/spermine acetyltransferase (2005), J. Biol. Chem., 280, 40328-40336.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.57 expression in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.57 hanging drop vapor diffusion method, crystal structure of PaiA in complex with CoA at 1.9A resolution, crystals belong to space group C222(1), with cell parameters of alpha = 39.9 A, beta = 135.8 A and gamma = 132.4 A Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.57 0.031
-
 acetyl-CoA pH 9 Bacillus subtilis
2.3.1.57 0.076
-
 spermine pH 9 Bacillus subtilis
2.3.1.57 0.295
-
 N1-acetylspermine pH 9 Bacillus subtilis
2.3.1.57 0.323
-
 spermidine pH 9 Bacillus subtilis
2.3.1.57 0.41
-
 aminopropylcadaverine pH 9 Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.57 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.57
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.57 acetyl-CoA + aminopropylcadaverine
-
 Bacillus subtilis ?
-
 ?
2.3.1.57 acetyl-CoA + N1-acetylspermine
-
 Bacillus subtilis CoA + N1,N12-diacetylspermine
-
 ?
2.3.1.57 acetyl-CoA + spermidine
-
 Bacillus subtilis CoA + N1-acetylspermidine
-
 ?
2.3.1.57 acetyl-CoA + spermine
-
 Bacillus subtilis CoA + N1-acetylspermine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.57 N1-spermidine/spermine acetyltransferase
-
 Bacillus subtilis
2.3.1.57 PaiA
-
 Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.57 0.087
-
 spermidine pH 9 Bacillus subtilis
2.3.1.57 0.1
-
 aminopropylcadaverine pH 9 Bacillus subtilis
2.3.1.57 0.12
-
 N1-acetylspermine pH 9 Bacillus subtilis
2.3.1.57 0.32
-
 spermine pH 9 Bacillus subtilis
2.3.1.57 0.365
-
 acetyl-CoA pH 9, cosubstrate: spermine Bacillus subtilis