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Literature summary extracted from

  • Oldham, M.L.; Brash, A.R.; Newcomer, M.E.
    Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via a C2-like domain and a structural basis of product chirality (2005), J. Biol. Chem., 280, 39545-39552.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.40 expressed in Escherichia coli BL21(DE3) cells Plexaura homomalla

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.40 hanging drop vapour diffusion in 6.25% polyethylene glycol 8000, 100 mM imidazole acetate (pH 8.0), 100 mM CaCl2, and 5% sucrose at 22°C Plexaura homomalla

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.40 D39A 118% activity compared to the wild type enzyme, mutant with diminished fluorescence resonance energy transfer properties, consistent with a role for calcium in membrane binding Plexaura homomalla
1.13.11.40 D39A/E47A 106% activity compared to the wild type enzyme, a double mutant with calcium-binding residues from two of the three sites mutated exhibits no fluorescence resonance energy transfer signal Plexaura homomalla
1.13.11.40 E47A 65% of the activity of the wild type enzyme, mutant with diminished fluorescence resonance energy transfer properties, consistent with a role for calcium in membrane binding Plexaura homomalla
1.13.11.40 W41A 140% activity compared to the wild type enzyme, exhibits only less than 2% of the increase in fluorescence at 517 nm upon the addition of CaCl2 of the wild-type signal Plexaura homomalla
1.13.11.40 W77A 44% of the activity of the wild type enzyme, exhibits only 4% of the increase in fluorescence at 517 nm upon the addition of CaCl2 of the wild-type signal Plexaura homomalla

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.40 Ca2+ Ca2+-regulated enzyme activity Plexaura homomalla

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.40 Plexaura homomalla
-
 Caribbean sea whip coral
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.40 nickel-nitrilotriacetic acid-agarose chromatography, DE52 column chromatography, and Mono Q column chromatography Plexaura homomalla

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.40 arachidonic acid + O2
-
 Plexaura homomalla (8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.40 8R-lipoxygenase
-
 Plexaura homomalla
1.13.11.40 8R-LOX
-
 Plexaura homomalla