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Literature summary extracted from

  • Zang, H.; Fang, Q.; Pegg, A.E.; Guengerich, F.P.
    Kinetic analysis of steps in the repair of damaged DNA by human O6-alkylguanine-DNA alkyltransferase (2005), J. Biol. Chem., 280, 30873-30881.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.63 expressed in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.63 R128G reduces the AGT repair efficiency, no smaller effects with the O6-benzylguanine substrate than the 6-O-methylguanine Homo sapiens
2.1.1.63 R128L reduces the AGT repair efficiency, smaller effects with the O6-benzylguanine substrate than the 6-O-methylguanine Homo sapiens
2.1.1.63 Y114F reduces the AGT repair efficiency, smaller effects with the O6-benzylguanine substrate than the 6-O-methylguanine Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.63 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.63
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.63 DNA containing 6-O-benzylguanine + [protein]-L-cysteine AGT binds and scans DNA rapidly, flips O6-alkylG residues, transfers the alkyl group in a chemical step that is not rate-limiting in the case of 6-O-benzylguanine and releases the dealkylated DNA Homo sapiens DNA lacking 6-O-benzylguanine + [protein]-S-methyl-L-cysteine
-
 ?
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine AGT binds and scans DNA rapidly, flips O6-alkylG residues, transfers the alkyl group in a chemical step that is rate-limiting in the case of 6-O-methylguanine and releases the dealkylated DNA Homo sapiens DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.63 AGT
-
 Homo sapiens
2.1.1.63 O6-alkylguanine-DNA alkyltransferase
-
 Homo sapiens