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Literature summary extracted from

  • Kitzing, K.; Fitzpatrick, T.B.; Wilken, C.; Sawa, J.; Bourenkov, G.P.; Macheroux, P.; Clausen, T.
    The 1.3 A crystal structure of the flavoprotein YqjM reveals a novel class of Old Yellow Enzymes (2005), J. Biol. Chem., 280, 27904-27913.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.6.5.10 sitting drop vapour diffusion method. In addition to the oxidized and reduced enzyme form, the structures of the complexes with p-hydroxybenzaldehyde and p-nitrophenol, respectively are solved Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.10 0.019
-
 duroquinone pH 7.5, 25°C Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.10 Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.10 NADPH + H+ + duroquinone
-
 Bacillus subtilis NADP+ + duroquinol
-
 ?
1.6.5.10 NADPH + H+ + menadione
-
 Bacillus subtilis NADP+ + reduced menadiol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.5.10 tetramer dimer of catalytically dependent dimers Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.5.10 2.67
-
 duroquinone pH 7.5, 25°C Bacillus subtilis
1.6.5.10 3.04
-
 menadione pH 7.5, 25°C Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.10 FMN bound non-covalently at the COOH termini of the beta-sheet Bacillus subtilis
1.6.5.10 NADPH
-
 Bacillus subtilis