Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chaudhary, K.; Donald, R.G.; Nishi, M.; Carter, D.; Ullman, B.; Roos, D.S.
    Differential localization of alternatively spliced hypoxanthine-xanthine-guanine phosphoribosyltransferase isoforms in Toxoplasma gondii (2005), J. Biol. Chem., 280, 22053-22059.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.8 gene HXGPRT, two isozymes, genetic organization, expression analysis of two alternative-splicing-derived isozymes, which differ in the presence or absence of a 49-amino acid insertion, specified by a single differentially spliced exon, but exhibit similar temporal expression patterns, isozyme expression in parasites lacking the endogenous hxgprt gene, expression of isozyme I in the cytosol and of isozyme II inthe inner membrane complex in enzyme-deficient parasites, co-expression of both isoforms results in the formation of heterooligomers, which distribute between the cytosol and IMC Toxoplasma gondii

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.8 additional information chimeric constructs expressing N-terminal peptides of 11 amino acids from isoform I or of 60 amino acids from isoform II fused to a chloramphenicol acetyl transferase reporter show that the N-terminal domain of isoform II is both necessary and sufficient for membrane association Toxoplasma gondii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.8 cytosol isozyme HXGPRT-I lacking the 49-amino acid insertion Toxoplasma gondii 5829
-
2.4.2.8 membrane isozyme HXGPRT-II possessisng the 49-amino acid insertion, localizes to the inner membrane complex (IMC) of the parasite Toxoplasma gondii 16020
-
2.4.2.8 additional information differential localization of alternatively spliced isozymes, overview Toxoplasma gondii
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Toxoplasma gondii
-
 IMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Toxoplasma gondii
-
 single gene locus, two alternative-splicing-derived isozymes HXGPRT-I and HXGPRT-II
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Toxoplasma gondii IMP + diphosphate
-
 ?
2.4.2.8 additional information the two isozymes exhibit similar substrate specificity, kinetic characteristics, and temporal expression patterns, overview Toxoplasma gondii ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.8 HXGPRT
-
 Toxoplasma gondii
2.4.2.8 hypoxanthine-xanthine-guanine phosphoribosyltransferase
-
 Toxoplasma gondii