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Literature summary extracted from

  • Rosenberg, M.F.; Kamis, A.B.; Aleksandrov, L.A.; Ford, R.C.; Riordan, J.R.
    Purification and crystallization of the cystic fibrosis transmembrane conductance regulator (CFTR) (2004), J. Biol. Chem., 279, 39051-39057.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.1.6 C terminally deca-histidine-tagged wild type human CFTR epxressed in baby hamster kidney-21 cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.6.1.6 by hanging drop method, at 20 A resolution in the xy plane, at 30 A resolution along z plane, two crystal forms, one with a roughly hexagonal profile, the other with an opened-out triangular profile, the two conformations are in presence of a nucleotide, which may be related to the role of CFTR as an ion channel rather than a transporter, and may represent the open and closed states of the channel Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.1.6 membrane
-
 Homo sapiens 16020
-

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.6 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.6 Ni-NTA matrix and subsequent WGA-agorse affinity step Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6.1.6 0.06
-
 with ATP as substrate Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.6 ATP + H2O + closed Cl- channel
-
 Homo sapiens ADP + phosphate + open Cl- channel
-
 ?
5.6.1.6 GTP + H2O + closed Cl- channel
-
 Homo sapiens GDP + phosphate + open Cl- channel
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.1.6 CFTR
-
 Homo sapiens
5.6.1.6 cystic fibrosis transmembrane conductance regulator
-
 Homo sapiens