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Literature summary extracted from
- Biosynthesis of riboflavin. Studies on the mechanism of L-3,4-dihydroxy-2-butanone 4-phosphate synthase (1991), J. Biol. Chem., 266, 20610-20618.
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.99.12 | Mg2+ | - |
Meyerozyma guilliermondii |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.99.12 | D-ribulose 5-phosphate | Meyerozyma guilliermondii | a step in the formation of the riboflavin precursor, 6,7-dimethyl-8-ribityllumazine, from 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione requiring a phosphorylated 4-carbon intermediate, designated as compound X, analysis of the riboflavin biosynthetic pathway, overview | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.99.12 | Meyerozyma guilliermondii | - |
i.e. Pichia guilliermondii | - |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 4.1.99.12 | D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate | catalytic mechanism | Meyerozyma guilliermondii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.99.12 | D-ribulose 5-phosphate | a step in the formation of the riboflavin precursor, 6,7-dimethyl-8-ribityllumazine, from 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione requiring a phosphorylated 4-carbon intermediate, designated as compound X, analysis of the riboflavin biosynthetic pathway, overview | Meyerozyma guilliermondii | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
| 4.1.99.12 | D-ribulose 5-phosphate | carbon atoms 1-3 of the enzyme product correspond to carbon atoms 1-3 of the substrate, whereas C-4 of the product stems from C-6 of the substrate. Carbon atom 4 of the substrate is released as formate together with the hydrogen atom attached to it. The skeletal rearrangement which leads to the loss of C-4 and the direct linkage between C-3 and C-6 of the substrate is an intramolecular reaction, the hydrogen atom at C-3 of the enzyme product is introduced from solvent water | Meyerozyma guilliermondii | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | product identification by GC-MS | ? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.99.12 | L-3,4-dihydroxy-2-butanone 4-phosphate synthase | - |
Meyerozyma guilliermondii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.12 | 37 | - |
assay at | Meyerozyma guilliermondii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.12 | 7 | - |
assay at | Meyerozyma guilliermondii |
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Release 2023.1 (January 2023)
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