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Literature summary extracted from

  • Asai, R.; Nishino, T.; Matsumura, T.; Okamoto, K.; Igarashi, K.; Pai, E.F.; Nishino, T.
    Two mutations convert mammalian xanthine oxidoreductase to highly superoxide-productive xanthine oxidase (2007), J. Biochem., 141, 525-534.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.17.1.4 mutant W335A/F336L, showing two similar, but not identical subunits. The cluster involved in conformation-switching is completely disrupted in one subunit, but remains partly associated in the other. Xanthine oxidase and oxidoreductase forms of the mutant are in equilibrium that greatly favors the oxidase form, but upon incubation with dithiothreitol equilibrium is partly shifted towards the oxidoreductase form Rattus norvegicus
1.17.3.2 xanthine oxidoreductase mutant W335A/F336L, showing two similar, but not identical subunits. The cluster involved in conformation-switching is completely disrupted in one subunit, but remains partly associated in the other. Xanthine oxidase and oxidoreductase forms of the mutant are in equilibrium that greatly favors the oxidase form, but upon incubation with dithiothreitol equilibrium is partly shifted towards the oxidoreductase form Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.17.1.4 W335A/F336L mutant oxidoreductase displaying xanthine oxidase activity Rattus norvegicus
1.17.3.2 W335A/F336L xanthine oxidoreductase mutant displaying xanthine oxidase activity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.3.2 0.129
-
 O2 xanthine oxidoreductase mutant W335A/F336L treated with dithiothreitol, pH 7.8, 25°C Rattus norvegicus
1.17.3.2 0.129
-
 O2 xanthine oxidoreductase mutant W335A/F336L, pH 7.8, 25°C Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Rattus norvegicus P22985 conversion of oxidoreductatse to oxidase
-
1.17.3.2 Rattus norvegicus P22985 conversion of xanthine oxidoreductase to oxidase
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 xanthine + NAD+ + H2O
-
 Rattus norvegicus urate + NADH + H+
-
 ?
1.17.3.2 xanthine + H2O + O2
-
 Rattus norvegicus urate + H2O2
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.17.3.2 0.41
-
 xanthine xanthine oxidoreductase mutant W335A/F336L, pH 7.8, 25°C Rattus norvegicus
1.17.3.2 9.73
-
 xanthine xanthine oxidoreductase mutant W335A/F336L treated with dithiothreitol, pH 7.8, 25°C Rattus norvegicus
1.17.3.2 12.47
-
 xanthine xanthine oxidoreductase mutant W335A/F336L, pH 7.8, 25°C Rattus norvegicus