Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Miller, J.R.; Ohren, J.; Sarver, R.W.; Mueller, W.T.; de Dreu, P.; Case, H.; Thanabal, V.
    Phosphopantetheine adenylyltransferase from Escherichia coli: Investigation of the kinetic mechanism and role in regulation of CoA biosynthesis (2007), J. Bacteriol., 189, 8196-8205.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.7.3 medicine PPAT is a target for antibacterial drug discovery Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.3 expression of His6-tagged and wild-type PPAT in Escherichia coli BL21(DE3) Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.3 at pH 5.0, 100 mM sodium acetate, pH 5.0, 1.1 M ammonium sulfate, 200 mM NaCl Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.3 R106A pantothenate kinase (PanK) mutant Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.3 CoA
-
 Escherichia coli
2.7.7.3 dephospho-CoA
-
 Escherichia coli
2.7.7.3 diphosphate
-
 Escherichia coli
2.7.7.3 additional information no inhibition is observed with AMPCPP, ATP-gamma-S, ADPPNP, or ADP at concentrations up to 1 mM and although no inhibition is observed with acetyl-CoA, desulfo-CoA, and palmitoyl-CoA at concentrations up to 500 mM Escherichia coli
2.7.7.3 phosphopantetheine
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.3 0.0047
-
 phosphopantetheine
-
 Escherichia coli
2.7.7.3 0.0047
-
 phosphopantetheine forward reaction Escherichia coli
2.7.7.3 0.017
-
 dephospho-CoA
-
 Escherichia coli
2.7.7.3 0.017
-
 dephospho-CoA reverse reaction Escherichia coli
2.7.7.3 0.22
-
 ATP
-
 Escherichia coli
2.7.7.3 0.22
-
 Mg-ATP forward reaction Escherichia coli
2.7.7.3 0.23
-
 diphosphate
-
 Escherichia coli
2.7.7.3 0.23
-
 diphosphate reverse reaction Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.3 phosphopantetheine + Mg-ATP Escherichia coli essential hexameric enzyme that catalyzes the penultimate step in coenzyme A biosynthesis dephospho-CoA + diphosphate
-
 r
2.7.7.3 phosphopantetheine + MgATP2- + 4 H+ Escherichia coli coenzyme A (CoA) biosynthesis dephospho-CoA + diphosphate + Mg2+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.3 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.3 copurification as hexamer with 0.5 equivalents of CoA tightly bound per PPAT monomer Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.3 ATP + pantetheine 4'-phosphate
-
 Escherichia coli diphosphate + 3'-dephospho-CoA
-
 ?
2.7.7.3 phosphopantetheine + Mg-ATP
-
 Escherichia coli dephospho-CoA + diphosphate
-
 r
2.7.7.3 phosphopantetheine + Mg-ATP essential hexameric enzyme that catalyzes the penultimate step in coenzyme A biosynthesis Escherichia coli dephospho-CoA + diphosphate
-
 r
2.7.7.3 phosphopantetheine + MgATP2- + 4 H+ coenzyme A (CoA) biosynthesis Escherichia coli dephospho-CoA + diphosphate + Mg2+
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.3 homohexamer 6*17800, the dyad axis of the hexamer divides the protein into two asymmetrical trimers Escherichia coli
2.7.7.3 homohexamer taken from literature, 6*17800Da Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.7.3 phosphopantetheine adenylyltransferase
-
 Escherichia coli
2.7.7.3 PPAT
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.3 0.0084
-
 phosphopantetheine Substrate: dephospho-CoA, Cosubstrate: diphosphate (1.6 mM) Escherichia coli
2.7.7.3 0.011
-
 CoA Substrate: dephospho-CoA, Cosubstrate: diphosphate (1.6 mM) Escherichia coli
2.7.7.3 0.014
-
 CoA Substrate: phosphopantetheine, Cosubstrate: ATP (300 microM) Escherichia coli
2.7.7.3 0.07
-
 CoA Substrate: ATP, Cosubstrate: phosphopantetheine (300 microM) Escherichia coli
2.7.7.3 0.117
-
 CoA Substrate: Pyrophosphate, Cosubstrate: dephospho-CoA (300 microM) Escherichia coli
2.7.7.3 0.12
-
 diphosphate Substrate: ATP, Cosubstrate: phosphopantetheine (300 microM) Escherichia coli
2.7.7.3 0.121
-
 dephospho-CoA Substrate: phosphopantetheine, Cosubstrate: ATP (3.0 mM) Escherichia coli
2.7.7.3 0.131
-
 phosphopantetheine Substrate: diphosphate, Cosubstrate: dephospho-CoA (500 microM) Escherichia coli
2.7.7.3 0.192
-
 dephospho-CoA Substrate: ATP, Cosubstrate: phosphopantetheine (300 microM) Escherichia coli