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Literature summary extracted from

  • Suzuki, H.; Ohnishi, Y.; Horinouchi, S.
    Arylamine N-acetyltransferase responsible for acetylation of 2-aminophenols in Streptomyces griseus (2007), J. Bacteriol., 189, 2155-2159.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.5 gene natA, DNA and amino acid sequence determination and analysis, genetic structure, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3) Streptomyces griseus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.5 additional information construction of a natA disruption mutant that is catalytically inactive Streptomyces griseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.5 0.81
-
 2-amino-4-chlorophenol pH 7.0, 30°C, recombinant enzyme Streptomyces griseus
2.3.1.5 1.4
-
 2-Aminophenol pH 7.0, 30°C, recombinant enzyme Streptomyces griseus
2.3.1.5 1.9
-
 2-amino-4-methylphenol pH 7.0, 30°C, recombinant enzyme Streptomyces griseus
2.3.1.5 2
-
 3-Amino-4-hydroxybenzoic acid pH 7.0, 30°C, recombinant enzyme Streptomyces griseus
2.3.1.5 2.6
-
 3-amino-4-hydroxybenzaldehyde pH 7.0, 30°C, recombinant enzyme Streptomyces griseus
2.3.1.5 12
-
 3-amino-4-hydroxybenzenesulfonic acid pH 7.0, 30°C, recombinant enzyme Streptomyces griseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.5 cytosol
-
 Streptomyces griseus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.5 34000
-
 1 * 34000, recombinant enzyme, SDS-PAGE Streptomyces griseus
2.3.1.5 40000
-
 recombinant enzyme, gel filtration Streptomyces griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.5 acetyl-CoA + 2-aminophenol Streptomyces griseus
-
 CoA + 2-acetylaminophenol
-
 ?
2.3.1.5 acetyl-CoA + 2-aminophenol Streptomyces griseus IFO 13350
-
 CoA + 2-acetylaminophenol
-
 ?
2.3.1.5 acetyl-CoA + 3-amino-4-hydroxybenzaldehyde Streptomyces griseus an intermediate of grixazone synthesis CoA + 3-acetylamino-4-hydroxybenzaldehyde
-
 ?
2.3.1.5 acetyl-CoA + 3-amino-4-hydroxybenzaldehyde Streptomyces griseus IFO 13350 an intermediate of grixazone synthesis CoA + 3-acetylamino-4-hydroxybenzaldehyde
-
 ?
2.3.1.5 acetyl-CoA + 3-amino-4-hydroxybenzoic acid Streptomyces griseus an intermediate of grixazone synthesis CoA + 3-acetylamino-4-hydroxybenzoic acid
-
 ?
2.3.1.5 acetyl-CoA + 5-aminosalicylic acid Streptomyces griseus
-
 CoA + 5-acetylaminosalicylic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.5 Streptomyces griseus A2V7M5 gene natA
-
2.3.1.5 Streptomyces griseus IFO 13350 A2V7M5 gene natA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.5 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Streptomyces griseus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.5 acetyl-CoA + an arylamine = CoA + an N-acetylarylamine Cys69, His108, and Asp125 form the catalytic triad Streptomyces griseus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.5 additional information
-
-
 Streptomyces griseus
2.3.1.5 0.29
-
 purified recombinant enzyme, with substrate 3-amino-4-hydroxybenzenesulfonic acid Streptomyces griseus
2.3.1.5 0.33
-
 purified recombinant enzyme, with substrate 3-amino-4-hydroxybenzaldehyde Streptomyces griseus
2.3.1.5 0.46
-
 purified recombinant enzyme, with substrate 2-amino-4-chlorophenol Streptomyces griseus
2.3.1.5 0.47
-
 purified recombinant enzyme, with substrate 2-aminophenol Streptomyces griseus
2.3.1.5 1.1
-
 purified recombinant enzyme, with substrate 3-amino-4-hydroxybenzoic acid or 2-amino-4-methylphenol Streptomyces griseus

Storage Stability

EC Number Storage Stability Organism
2.3.1.5 4°C, purified recombinant enzyme in 50 mM sodium phosphate, pH 7.0, 0.15 M NaCl, and 7.5 mM 2-mercaptoethanol, 2 weeks, stable Streptomyces griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.5 acetyl-CoA + 2-amino-4-chlorophenol
-
 Streptomyces griseus CoA + 2-acetylamino-4-chlorophenol
-
 ?
2.3.1.5 acetyl-CoA + 2-amino-4-methylphenol best substrate Streptomyces griseus CoA + 2-acetylamino-4-methylphenol
-
 ?
2.3.1.5 acetyl-CoA + 2-aminophenol
-
 Streptomyces griseus CoA + 2-acetylaminophenol
-
 ?
2.3.1.5 acetyl-CoA + 2-aminophenol
-
 Streptomyces griseus IFO 13350 CoA + 2-acetylaminophenol
-
 ?
2.3.1.5 acetyl-CoA + 3-amino-4-hydroxybenzaldehyde
-
 Streptomyces griseus CoA + 3-acetylamino-4-hydroxybenzaldehyde
-
 ?
2.3.1.5 acetyl-CoA + 3-amino-4-hydroxybenzaldehyde an intermediate of grixazone synthesis Streptomyces griseus CoA + 3-acetylamino-4-hydroxybenzaldehyde
-
 ?
2.3.1.5 acetyl-CoA + 3-amino-4-hydroxybenzaldehyde
-
 Streptomyces griseus IFO 13350 CoA + 3-acetylamino-4-hydroxybenzaldehyde
-
 ?
2.3.1.5 acetyl-CoA + 3-amino-4-hydroxybenzaldehyde an intermediate of grixazone synthesis Streptomyces griseus IFO 13350 CoA + 3-acetylamino-4-hydroxybenzaldehyde
-
 ?
2.3.1.5 acetyl-CoA + 3-amino-4-hydroxybenzenesulfonic acid
-
 Streptomyces griseus CoA + 3-acetylamino-4-hydroxybenzenesulfonic acid
-
 ?
2.3.1.5 acetyl-CoA + 3-amino-4-hydroxybenzoic acid best substrate Streptomyces griseus CoA + 3-acetylamino-4-hydroxybenzoic acid
-
 ?
2.3.1.5 acetyl-CoA + 3-amino-4-hydroxybenzoic acid an intermediate of grixazone synthesis Streptomyces griseus CoA + 3-acetylamino-4-hydroxybenzoic acid
-
 ?
2.3.1.5 acetyl-CoA + 3-amino-4-hydroxybenzoic acid best substrate Streptomyces griseus IFO 13350 CoA + 3-acetylamino-4-hydroxybenzoic acid
-
 ?
2.3.1.5 acetyl-CoA + 4-amino-3-hydroxybenzoic acid low activity Streptomyces griseus CoA + 4-acetylamino-3-hydroxybenzoic acid
-
 ?
2.3.1.5 acetyl-CoA + 5-aminosalicylic acid
-
 Streptomyces griseus CoA + 5-acetylaminosalicylic acid
-
 ?
2.3.1.5 additional information substrate specificity, product identification by LC-MS, 4-aminobenzoic acid, 3-aminobenzoic acid, and aniline are poor substrates, overview Streptomyces griseus ?
-
 ?
2.3.1.5 additional information substrate specificity, product identification by LC-MS, 4-aminobenzoic acid, 3-aminobenzoic acid, and aniline are poor substrates, overview Streptomyces griseus IFO 13350 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.5 monomer 1 * 34000, recombinant enzyme, SDS-PAGE Streptomyces griseus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.5 NAT
-
 Streptomyces griseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.5 30
-
 assay at Streptomyces griseus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.5 0.27
-
 2-amino-4-chlorophenol pH 7.0, 30°C, recombinant enzyme Streptomyces griseus
2.3.1.5 0.29
-
 3-amino-4-hydroxybenzaldehyde pH 7.0, 30°C, recombinant enzyme Streptomyces griseus
2.3.1.5 0.41
-
 2-Aminophenol pH 7.0, 30°C, recombinant enzyme Streptomyces griseus
2.3.1.5 1.1
-
 3-amino-4-hydroxybenzenesulfonic acid pH 7.0, 30°C, recombinant enzyme Streptomyces griseus
2.3.1.5 1.3
-
 3-Amino-4-hydroxybenzoic acid pH 7.0, 30°C, recombinant enzyme Streptomyces griseus
2.3.1.5 1.3
-
 2-amino-4-methylphenol pH 7.0, 30°C, recombinant enzyme Streptomyces griseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.5 7
-
 assay at Streptomyces griseus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.5 acetyl-CoA
-
 Streptomyces griseus