Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Murakami, T.; Kanai, T.; Takata, H.; Kuriki, T.; Imanaka, T.
    A novel branching enzyme of the GH-57 family in the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 (2006), J. Bacteriol., 188, 5915-5924.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.18 expressed in Escherichia coli Bl21-CodonPlus(DE3)-RIL and pET21a(+) Thermococcus kodakarensis

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.18 additional information recombinant TK1436 protein (amino acids [aa] 1 to 675) and a deletion derivative devoid of the C-terminal two-copy helix-hairpin-helix (HhH)2 motif (TK1436deltaH, aa 1 to 562) Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.18 78550
-
 calculated molecular mass of the TK1436 protein Thermococcus kodakarensis
2.4.1.18 558000
-
 TK1436deltaH protein, gel filtration on a Superdex 200 HR 10/30 column Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.18 Thermococcus kodakarensis Q5JDJ7 TK1436 encodes a protein of 675 aa; hyperthermophilic archaeon
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.18 anion exchange column and hydrophobic column Thermococcus kodakarensis

Storage Stability

EC Number Storage Stability Organism
2.4.1.18 the TK1436 protein shows rapid degradation within 2 to 3 days of storage at 4°C, while the TK1436deltaH protein is stable unter the same conditions Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.18 TK1436 encodes a branching enzyme from Thermococcus kodakaraensis belonging to the GH-57 family. The TK1436 protein contains neither alpha-galactosidase nor pullulanase activity. Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.18 70
-
 determined by iodine-staining assay Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.18 90
-
 the protein is stable up to 90°C, the activity decreases gradually during the incubation at 100°C Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.18 7
-
 determined by iodine-staining assay Thermococcus kodakarensis