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Literature summary extracted from
- Identification and characterization of bacterial cysteine dioxygenases: a new route of cysteine degradation for eubacteria (2006), J. Bacteriol., 188, 5561-5569.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.13.11.20 | - |
Rattus norvegicus |
| 1.13.11.20 | the open reading frame of putative cysteine dioxygenase NP_390992(yubC, Bacillus subtilis) is cloned and overexpressed in Escherichia coli. | Bacillus subtilis |
| 1.13.11.20 | the open reading frame of putative cysteine dioxygenase NP_627257 (SCO30335, Streptomyces coelicolor A3(2))is cloned and overexpressed in Escherichia coli | Streptomyces coelicolor |
| 1.13.11.20 | the open reading frame of putative cysteine dioxygenase NP_629897 (SCO5772, Streptomyces coelicolor A3(2)) is cloned and overexpressed in Escherichia coli | Streptomyces coelicolor |
| 1.13.11.20 | the open reading frame of putative cysteine dioxygenase NP_832375(BC2617, Bacillus cereus ATCC 14579) is cloned and overexpressed in Escherichia coli | Bacillus cereus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.20 | 2-amino-ethanethiol | 1 x the Km for cysteine = -2.5% inhibition, 10 x the Km for cysteine = 20% inhibition | Bacillus cereus |  |
| 1.13.11.20 | 2-amino-ethanethiol | YubC: 1 x the Km for cysteine = 12% inhibition, 10 x the Km for cysteine = 19% inhibition | Bacillus subtilis | |
| 1.13.11.20 | 2-amino-ethanethiol | 1 x the Km for cysteine = 8.7% inhibition, 10 x the Km for cysteine = 30% inhibition | Rattus norvegicus | |
| 1.13.11.20 | 2-amino-ethanethiol | SCO3035: 1 x the Km for cysteine = 7.5% inhibition, 10 x the Km for cysteine = 27% inhibition; SCO5772: 1 x the Km for cysteine = 0.9% inhibition, 10 x the Km for cysteine = 28% inhibition | Streptomyces coelicolor | |
| 1.13.11.20 | 2-sulfanyl-ethanol | 1 x the Km for cysteine = 4.8% inhibition, 10 x the Km for cysteine = 11% inhibition | Bacillus cereus | |
| 1.13.11.20 | 2-sulfanyl-ethanol | YubC: 1 x the Km for cysteine = -0.7% inhibition, 10 x the Km for cysteine = 6.6% inhibition | Bacillus subtilis | |
| 1.13.11.20 | 2-sulfanyl-ethanol | 1 x the Km for cysteine = 5.9% inhibition, 10 x the Km for cysteine = 13% inhibition | Rattus norvegicus | |
| 1.13.11.20 | 2-sulfanyl-ethanol | SCO3035: 1 x the Km for cysteine = 8.6% inhibition, 10 x the Km for cysteine = 15% inhibition; SCO5772: 1 x the Km for cysteine = 2.6% inhibition, 10 x the Km for cysteine = 13% inhibition | Streptomyces coelicolor | |
| 1.13.11.20 | 3-sulfanyl-propionic acid | 1 x the Km for cysteine = 3.2% inhibition, 10 x the Km for cysteine = 12% inhibition | Bacillus cereus | |
| 1.13.11.20 | 3-sulfanyl-propionic acid | YubC: 1 x the Km for cysteine = 4.7% inhibition, 10 x the Km for cysteine = 11% inhibition | Bacillus subtilis | |
| 1.13.11.20 | 3-sulfanyl-propionic acid | 1 x the Km for cysteine = 4.9% inhibition, 10 x the Km for cysteine = 26% inhibition | Rattus norvegicus | |
| 1.13.11.20 | 3-sulfanyl-propionic acid | SCO3035: 1 x the Km for cysteine = 2.9% inhibition, 10 x the Km for cysteine = 11% inhibition; SCO5772: 1 x the Km for cysteine = 5.6% inhibition, 10 x the Km for cysteine = 16% inhibition | Streptomyces coelicolor | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.20 | 1.2 | - |
L-cysteine | SCO3035, measurement of cysteine sulfinic acid production is done by high-performance liquid chromatography | Streptomyces coelicolor | |
| 1.13.11.20 | 1.5 | - |
L-cysteine | measurement of cysteine sulfinic acid production is done by high-performance liquid chromatography | Rattus norvegicus | |
| 1.13.11.20 | 3 | - |
cysteine | YubC, measurement of cysteine sulfinic acid production is done by high-performance liquid chromatography | Bacillus subtilis | |
| 1.13.11.20 | 3.8 | - |
L-cysteine | SCO5772, measurement of cysteine sulfinic acid production is done by high-performance liquid chromatography | Streptomyces coelicolor | |
| 1.13.11.20 | 5.7 | - |
L-cysteine | measurement of cysteine sulfinic acid production is done by high-performance liquid chromatography | Bacillus cereus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.20 | Fe2+ | upon purification the protein is catalytically inactive, activity can be restored upon the addition of exogenous ferrous iron, with maximal activation at a ferrous iron concentration of 0.3 mM | Streptomyces coelicolor | |
| 1.13.11.20 | Fe2+ | upon purification the protein is catalytically inactive, activity can be restored upon the addition of exogenous ferrous iron, with maximal activation at a ferrous iron concentration of 0.3 mM | Bacillus cereus | |
| 1.13.11.20 | Fe2+ | YubC: Upon purification the protein is catalytically inactive, activity can be restored upon the addition of exogenous ferrous iron, with maximal activation at a ferrous iron concentration of 0.3 mM | Bacillus subtilis | |
| 1.13.11.20 | additional information | Co2+ fails to restore significant activity to purified proteins | Streptomyces coelicolor | |
| 1.13.11.20 | additional information | Co2+ fails to restore significant activity to purified proteins | Bacillus cereus | |
| 1.13.11.20 | additional information | Mn2+ fails to restore significant activity to purified proteins | Streptomyces coelicolor | |
| 1.13.11.20 | additional information | Mn2+ fails to restore significant activity to purified proteins | Bacillus cereus | |
| 1.13.11.20 | additional information | Ni2+ fails to restore significant activity to purified proteins | Streptomyces coelicolor | |
| 1.13.11.20 | additional information | Ni2+ fails to restore significant activity to purified proteins | Bacillus cereus | |
| 1.13.11.20 | additional information | YubC: Co2+ fails to restore significant activity to purified proteins | Bacillus subtilis | |
| 1.13.11.20 | additional information | YubC: Mn2+ fails to restore significant activity to purified proteins | Bacillus subtilis | |
| 1.13.11.20 | additional information | YubC: Ni2+ fails to restore significant activity to purified proteins | Bacillus subtilis | |
| 1.13.11.20 | additional information | YubC: Zn2+ fails to restore significant activity to purified proteins | Bacillus subtilis | |
| 1.13.11.20 | additional information | Zn2+ fails to restore significant activity to purified proteins | Streptomyces coelicolor | |
| 1.13.11.20 | additional information | Zn2+ fails to restore significant activity to purified proteins | Bacillus cereus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.20 | Bacillus cereus | Q81CX4 | BC2617 has 13.0% sequence identity with rat CDO, calculated by a BLASTP alignment using VEctor NTI; BC2617 | - |
| 1.13.11.20 | Bacillus cereus DSM 31 | Q81CX4 | BC2617 has 13.0% sequence identity with rat CDO, calculated by a BLASTP alignment using VEctor NTI; BC2617 | - |
| 1.13.11.20 | Bacillus subtilis | O32085 | YubC has 18.7% sequence identity with rat CDO, calculated by a BLASTP alignment using Vector NTI; wild-type strain 168 and strain 168 YubC::pMUTIN4 and yubC, CDO activity is detectable in wild-type Bacillus subtilis during vegetative growth and increses to ~350% 10 h after sporulation induction. Neither yubC gene expression nor CDO catalytic activity can be detected in the yubC::pMUTIN4 strain. | - |
| 1.13.11.20 | no activity in Nostoc sp. | - |
strain PCC73102 | - |
| 1.13.11.20 | Rattus norvegicus | P21816 | - |
- |
| 1.13.11.20 | Streptomyces coelicolor | O50490 | SCO5772 has 16.4% sequence identity with rat CDO, calculated by a BLASTP alignment using Vector NTI; A3(2) SCO5772 | - |
| 1.13.11.20 | Streptomyces coelicolor | Q9KZL0 | SCO3035 has 20.6% sequence identity with rat CDO, calculated by a BLASTP alignment using VEctor NTI; A3(2) SCO3035 | - |
| 1.13.11.20 | Streptomyces coelicolor A3(2) SCO3035 | Q9KZL0 | SCO3035 has 20.6% sequence identity with rat CDO, calculated by a BLASTP alignment using VEctor NTI; A3(2) SCO3035 | - |
| 1.13.11.20 | Streptomyces coelicolor A3(2) SCO5772 | O50490 | SCO5772 has 16.4% sequence identity with rat CDO, calculated by a BLASTP alignment using Vector NTI; A3(2) SCO5772 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.20 | - |
Rattus norvegicus |
| 1.13.11.20 | HisTrap HP column, immobilized metal affinity chromatography | Streptomyces coelicolor |
| 1.13.11.20 | HisTrap HP column, immobilized metal affinity chromatography, during purification of SCO30335 two peaks of highly purified recombinant protein are found to elute: one at 10% (peak1) and the second at 20% (peak 2), kinetic data show that peak 1 has lower Km and higher Vmax values for cysteine than peak 2 | Streptomyces coelicolor |
| 1.13.11.20 | HisTrap HP column, immobilized metal affinity chromatography, during purification two peaks of highly purified recombinant protein are found to elute: one at 10% (peak1) and the second at 20% (peak 2), kinetic data show that peak 1 has lower Km and higher Vmax values for cysteine than peak 2 | Bacillus cereus |
| 1.13.11.20 | HisTrap HP column, immobilized metal affinity chromatography, during purification two peaks of highly purified recombinant protein are found to elute: one at 10% (peak1) and the second at 20%(peak 2), kinetic datat show that peak 1 has lower Km and higher Vmax values for cysteine thant peak 2. | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.20 | 0.7 | - |
SCO5772 | Streptomyces coelicolor |
| 1.13.11.20 | 0.8 | - |
SCO3035 | Streptomyces coelicolor |
| 1.13.11.20 | 1 | - |
YubC | Bacillus subtilis |
| 1.13.11.20 | 1.4 | - |
- |
Rattus norvegicus |
| 1.13.11.20 | 4.4 | - |
- |
Bacillus cereus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.20 | L-cysteine + O2 | - |
Streptomyces coelicolor | 3-sulfino-L-alanine | - |
? | |
| 1.13.11.20 | L-cysteine + O2 | - |
Bacillus subtilis | 3-sulfino-L-alanine | - |
? | |
| 1.13.11.20 | L-cysteine + O2 | - |
Bacillus cereus | 3-sulfino-L-alanine | - |
? | |
| 1.13.11.20 | L-cysteine + O2 | - |
Rattus norvegicus | 3-sulfino-L-alanine | - |
? | |
| 1.13.11.20 | L-cysteine + O2 | - |
Streptomyces coelicolor A3(2) SCO3035 | 3-sulfino-L-alanine | - |
? | |
| 1.13.11.20 | L-cysteine + O2 | - |
Bacillus cereus DSM 31 | 3-sulfino-L-alanine | - |
? | |
| 1.13.11.20 | L-cysteine + O2 | - |
Streptomyces coelicolor A3(2) SCO5772 | 3-sulfino-L-alanine | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.11.20 | CDO | - |
no activity in Nostoc sp. |
| 1.13.11.20 | CDO | - |
Streptomyces coelicolor |
| 1.13.11.20 | CDO | - |
Bacillus subtilis |
| 1.13.11.20 | CDO | - |
Bacillus cereus |
| 1.13.11.20 | CDO | - |
Rattus norvegicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.20 | 0.3 | - |
L-cysteine | SCO5772 | Streptomyces coelicolor | |
| 1.13.11.20 | 0.33 | - |
L-cysteine | SCO3035 | Streptomyces coelicolor | |
| 1.13.11.20 | 0.39 | - |
L-cysteine | YubC | Bacillus subtilis | |
| 1.13.11.20 | 0.62 | - |
L-cysteine | - |
Rattus norvegicus | |
| 1.13.11.20 | 2 | - |
L-cysteine | - |
Bacillus cereus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.20 | 6 | - |
SCO3035, pH optimum is determined by using 2-morpholinoethanesulfonic acid or Tris buffers at a final concentration of 62.5 mM | Streptomyces coelicolor |
| 1.13.11.20 | 6.1 | - |
pH optimum is determined by using 2-morpholinoethanesulfonic acid or Tris buffers at a final concentration of 62.5 mM | Bacillus cereus |
| 1.13.11.20 | 6.1 | - |
pH optimum is determined by using 2-morpholinoethanesulfonic acid or Tris buffers at a final concentration of 62.5 mM | Rattus norvegicus |
| 1.13.11.20 | 6.1 | - |
SCO5772, pH optimum is determined by using 2-morpholinoethanesulfonic acid or Tris buffers at a final concentration of 62.5 mM | Streptomyces coelicolor |
| 1.13.11.20 | 6.2 | - |
YubC, pH optimum is determined by using 2-morpholinoethanesulfonic acid or Tris buffers at a final concentration of 62.5 mM | Bacillus subtilis |
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