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Literature summary extracted from

  • Yang, K.; Eyobo, Y.; Brand, L.A.; Martynowski, D.; Tomchick, D.; Strauss, E.; Zhang, H.
    Crystal structure of a type III pantothenate kinase: insight into the mechanism of an essential coenzyme A biosynthetic enzyme universally distributed in bacteria (2006), J. Bacteriol., 188, 5532-5540.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.33 expressed in Escherichia coli strain BL21(DE3) Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.33 sitting drop vapour diffusion method with 15% polyethylene glycol 3350 Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.33 D105E less than 6% activity of the wild type enzyme Thermotoga maritima
2.7.1.33 D105N less than 6% activity of the wild type enzyme Thermotoga maritima
2.7.1.33 D125E less than 6% activity of the wild type enzyme Thermotoga maritima
2.7.1.33 D125N less than 6% activity of the wild type enzyme Thermotoga maritima
2.7.1.33 D6E less than 6% activity of the wild type enzyme Thermotoga maritima
2.7.1.33 D6N less than 6% activity of the wild type enzyme Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.33 additional information not inhibited by CoA or its thioesters Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.33 0.043
-
 (R)-pantothenate in 100 mM HEPES, pH 7.6, 20 mM KCl, 10 mM MgCl2, 2 mM phosphoenolpyruvate, 0.3 mM NADH, 5 units of lactate dehydrogenase, 2.5 units of pyruvate kinase, and 0.00027 mM of the PanK-III protein, at 50°C Thermotoga maritima
2.7.1.33 6.04
-
 ATP in 100 mM HEPES, pH 7.6, 20 mM KCl, 10 mM MgCl2, 2 mM phosphoenolpyruvate, 0.3 mM NADH, 5 units of lactate dehydrogenase, 2.5 units of pyruvate kinase, and 0.00027 mM of the PanK-III protein, at 50°C Thermotoga maritima

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.33 nickel-nitrilotriacetic acid-agarose column chromatography and Resource Q column chromatography Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate
-
 Thermotoga maritima ADP + (R)-4'-phosphopantothenate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.33 dimer gel filtration, functional unit in solution Thermotoga maritima
2.7.1.33 hexamer X-ray crystallography, in the crystal form Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
2.7.1.33 PanK-III
-
 Thermotoga maritima
2.7.1.33 type III pantothenate kinase
-
 Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.33 0.29
-
 (R)-pantothenate in 100 mM HEPES, pH 7.6, 20 mM KCl, 10 mM MgCl2, 2 mM phosphoenolpyruvate, 0.3 mM NADH, 5 units of lactate dehydrogenase, 2.5 units of pyruvate kinase, and 0.00027 mM of the PanK-III protein, at 50°C Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.33 ATP
-
 Thermotoga maritima