Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Safo, M.K.; Musayev, F.N.; di Salvo, M.L.; Hunt, S.; Claude, J.B.; Schirch, V.
    Crystal structure of pyridoxal kinase from the Escherichia coli pdxK gene: implications for the classification of pyridoxal kinases (2006), J. Bacteriol., 188, 4542-4552.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.35 expressed in Escherichia coli Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.35 hanging drop vapour diffusion method using 20 mM potassium phosphate, pH 7.5, 5 mM beta-mercaptoethanol, 0.2 mM EDTA, and 21% PEG 4K, 100 mM Tris-HCl, pH 8.5, 200 mM Na acetate, 40 mM MgSO4, and 10% glycerol Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.35 K+ in the absence of any potassium ion, the activity is less than 5% of the maximum activity Escherichia coli
2.7.1.35 Mg2+ required Escherichia coli
2.7.1.35 additional information the presence of 100 mM NaCl does not alter the potassium activation profile Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.35 Escherichia coli P40191
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.35
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.35 ATP + pyridoxine
-
 Escherichia coli ADP + pyridoxine 5'-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.35 ePL kinase 1
-
 Escherichia coli
2.7.1.35 PdxK
-
 Escherichia coli
2.7.1.35 pyridoxal kinase 1
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.35 ATP
-
 Escherichia coli