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Literature summary extracted from

  • Grochowski, L.L.; Xu, H.; White, R.H.
    Identification of lactaldehyde dehydrogenase in Methanocaldococcus jannaschii and its involvement in production of lactate for F420 biosynthesis (2006), J. Bacteriol., 188, 2836-2844.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.22 expression in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.22 iodacetamide
-
 Methanocaldococcus jannaschii
1.2.1.22 N-ethylmaleimide
-
 Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.22 0.284
-
 propionaldehyde 100 mM TES pH 7.5, 2.5 mM NAD+ Methanocaldococcus jannaschii
1.2.1.22 0.302
-
 D-Lactaldehyde 100 mM TES pH 7.5, 2.5 mM NAD+ Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.22 51000
-
 SDS-PAGE Methanocaldococcus jannaschii
1.2.1.22 180000
-
 gel-filtration Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.22 Methanocaldococcus jannaschii Q58806
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.22 anion-exchange chromatography Methanocaldococcus jannaschii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.22 cell culture
-
 Methanocaldococcus jannaschii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.22 0.6
-
 100 mM TES pH 7.5, 2.5 mM NAD+ Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.22 acetaldehyde + NAD+ + H2O 25% activity compared to D-lactaldehyde Methanocaldococcus jannaschii acetate + NADH + H+
-
 ?
1.2.1.22 acrylaldehyde + NAD+ + H2O 19% activity compared to D-lactaldehyde Methanocaldococcus jannaschii acrylate + NADH
-
 ?
1.2.1.22 crotonaldehyde + NAD+ + H2O 84% activity compared to D-lactaldehyde Methanocaldococcus jannaschii crotonate + NADH
-
 ?
1.2.1.22 D-lactaldehyde + NAD+ + H2O
-
 Methanocaldococcus jannaschii D-lactate + NADH + H+
-
 ?
1.2.1.22 DL-glyceraldehyde + NAD+ + H2O 97% activity compared to D-lactaldehyde Methanocaldococcus jannaschii glycerate + NADH
-
 ?
1.2.1.22 formaldehyde + NAD+ + H2O 4% activity compared to D-lactaldehyde Methanocaldococcus jannaschii formate + NADH
-
 ?
1.2.1.22 glycolaldehyde + NAD+ + H2O 35% activity compared to D-lactaldehyde Methanocaldococcus jannaschii glycolate + NADH
-
 ?
1.2.1.22 propionaldehyde + NAD+ + H2O 187% activity compared to D-lactaldehyde Methanocaldococcus jannaschii propionate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.22 tetramer 4 * 51000, gel-filtration Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
1.2.1.22 Lactaldehyde dehydrogenase
-
 Methanocaldococcus jannaschii
1.2.1.22 MJ1411
-
 Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.22 80
-
 86% activity after 80°C for 10 min Methanocaldococcus jannaschii
1.2.1.22 90
-
 45% activity after 90°C for 10 min Methanocaldococcus jannaschii
1.2.1.22 100
-
 25% activity after 100°C for 10 min Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.22 2
-
 D-Lactaldehyde 100 mM TES pH 7.5, 2.5 mM NAD+ Methanocaldococcus jannaschii
1.2.1.22 3
-
 propionaldehyde 100 mM TES pH 7.5, 2.5 mM NAD+ Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.22 9
-
-
 Methanocaldococcus jannaschii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.22 7 10 17% activity at pH 7.0, almost 100% activity at pH 10.0 Methanocaldococcus jannaschii