Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nishimasu, H.; Fushinobu, S.; Shoun, H.; Wakagi, T.
    Identification and characterization of an ATP-dependent hexokinase with broad substrate specificity from the hyperthermophilic archaeon Sulfolobus tokodaii (2006), J. Bacteriol., 188, 2014-2019.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.1 expression in Escherichia coli Sulfurisphaera tokodaii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 ADP 1 mM, 92% inhibition. Competitive inhibition versus ATP, noncompetitive inhibition versus D-glucose Sulfurisphaera tokodaii
2.7.1.1 AMP 1 mM, 73% inhibition Sulfurisphaera tokodaii
2.7.1.1 CDP 1 mM, 33% inhibition Sulfurisphaera tokodaii
2.7.1.1 D-fructose 6-phosphate 10 mM, 33% inhibition Sulfurisphaera tokodaii
2.7.1.1 diphosphate 10 mM, 54% inhibition Sulfurisphaera tokodaii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.05
-
 D-glucose pH 7.5, 50°C, ATP as phosphoryl donor Sulfurisphaera tokodaii
2.7.1.1 0.12
-
 ATP pH 7.5, 50°C, D-glucose as phosphoryl acceptor Sulfurisphaera tokodaii
2.7.1.1 0.13
-
 D-mannose pH 7.5, 50°C, ATP as phosphoryl donor Sulfurisphaera tokodaii
2.7.1.1 0.23
-
 D-glucosamine pH 7.5, 50°C, ATP as phosphoryl donor Sulfurisphaera tokodaii
2.7.1.1 0.32
-
 N-acetyl-D-glucosamine pH 7.5, 50°C, ATP as phosphoryl donor Sulfurisphaera tokodaii
2.7.1.1 0.44
-
 2-deoxy-D-glucose pH 7.5, 50°C, ATP as phosphoryl donor Sulfurisphaera tokodaii
2.7.1.1 0.52
-
 CTP pH 7.5, 50°C, D-glucose as phosphoryl acceptor Sulfurisphaera tokodaii
2.7.1.1 30
-
 D-fructose pH 7.5, 50°C, ATP as phosphoryl donor Sulfurisphaera tokodaii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.1 Co2+ no activity is detected without a divalent cation. 100, 153, and 80% relative activity in the presence of Mg2+, Co2+, and Mn2+, respectively, at 4 mM Sulfurisphaera tokodaii
2.7.1.1 Mg2+ no activity is detected without a divalent cation. 100, 153, and 80% relative activity in the presence of Mg2+, Co2+, and Mn2+, respectively, at 4 mM Sulfurisphaera tokodaii
2.7.1.1 Mn2+ no activity is detected without a divalent cation. 100, 153, and 80% relative activity in the presence of Mg2+, Co2+, and Mn2+, respectively, at 4 mM Sulfurisphaera tokodaii
2.7.1.1 additional information no activity in the presence of Zn2+ Sulfurisphaera tokodaii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.1 32000
-
 2 * 32000, SDS-PAGE Sulfurisphaera tokodaii
2.7.1.1 32100
-
 2 * 32100, calculated from sequence Sulfurisphaera tokodaii
2.7.1.1 64000
-
 gel filtration Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.1 ATP + D-glucose Sulfurisphaera tokodaii in Sulfolobus tokodaii this is the sole enzyme responsible for the phosphorylation of glucose in vivo ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 ATP + D-glucose Sulfurisphaera tokodaii 7 in Sulfolobus tokodaii this is the sole enzyme responsible for the phosphorylation of glucose in vivo ADP + D-glucose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Sulfurisphaera tokodaii Q96Y14
-
-
2.7.1.1 Sulfurisphaera tokodaii 7 Q96Y14
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1 recombinant enzyme Sulfurisphaera tokodaii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.1 26
-
 pH 7.5, 50°C, substrate: D-glucose Sulfurisphaera tokodaii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 ATP + 2-deoxy-D-glucose
-
 Sulfurisphaera tokodaii ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
2.7.1.1 ATP + 2-deoxy-D-glucose
-
 Sulfurisphaera tokodaii 7 ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
2.7.1.1 ATP + D-fructose
-
 Sulfurisphaera tokodaii ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 ATP + D-glucosamine
-
 Sulfurisphaera tokodaii ADP + D-glucosamine 6-phosphate
-
 ?
2.7.1.1 ATP + D-glucose
-
 Sulfurisphaera tokodaii ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 ATP + D-glucose in Sulfolobus tokodaii this is the sole enzyme responsible for the phosphorylation of glucose in vivo Sulfurisphaera tokodaii ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 ATP + D-glucose
-
 Sulfurisphaera tokodaii 7 ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 ATP + D-glucose in Sulfolobus tokodaii this is the sole enzyme responsible for the phosphorylation of glucose in vivo Sulfurisphaera tokodaii 7 ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 ATP + D-mannose
-
 Sulfurisphaera tokodaii ADP + D-mannose 6-phosphate
-
 ?
2.7.1.1 ATP + N-acetyl-D-glucosamine
-
 Sulfurisphaera tokodaii ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
2.7.1.1 CTP + D-glucose 81% of the activity with ATP Sulfurisphaera tokodaii CDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 GTP + D-glucose 52% of the activity with ATP Sulfurisphaera tokodaii GDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 ITP + D-glucose 64% of the activity with ATP Sulfurisphaera tokodaii IDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 ITP + D-glucose 64% of the activity with ATP Sulfurisphaera tokodaii 7 IDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 additional information galactose is phosphorylated at rates of 0.8 and 0.08 U/mg at concentrations of 1 and 10 mM, respectively. Ribose, xylose, N-acetylmannosamine, glucose-1-phosphate, and fructose-6-phosphate (10 mM) are not phosphorylated by the enzyme Sulfurisphaera tokodaii ?
-
 ?
2.7.1.1 additional information galactose is phosphorylated at rates of 0.8 and 0.08 U/mg at concentrations of 1 and 10 mM, respectively. Ribose, xylose, N-acetylmannosamine, glucose-1-phosphate, and fructose-6-phosphate (10 mM) are not phosphorylated by the enzyme Sulfurisphaera tokodaii 7 ?
-
 ?
2.7.1.1 UTP + D-glucose 37% of the activity with ATP Sulfurisphaera tokodaii UDP + D-glucose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.1 dimer 2 * 32000, SDS-PAGE Sulfurisphaera tokodaii
2.7.1.1 dimer 2 * 32100, calculated from sequence Sulfurisphaera tokodaii

Synonyms

EC Number Synonyms Comment Organism
2.7.1.1 ATP-dependent hexokinase
-
 Sulfurisphaera tokodaii
2.7.1.1 STK_23540 locus name Sulfurisphaera tokodaii
2.7.1.1 StoHK
-
 Sulfurisphaera tokodaii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.1 80
-
 assay at Sulfurisphaera tokodaii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.1 80
-
 pH 7.5, 120 min, 14% loss of activity Sulfurisphaera tokodaii
2.7.1.1 90
-
 pH 7.5, 120 min, 80% loss of activity Sulfurisphaera tokodaii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.1 7.5
-
-
 Sulfurisphaera tokodaii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.1 6.5 9 more than 60% of the maximal activity in the pH range of 6.5 to 9.0 Sulfurisphaera tokodaii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.1 0.018
-
 ADP pH 7.5, 50°C Sulfurisphaera tokodaii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.1 1.4
-
 D-fructose pH 7.5, 50°C Sulfurisphaera tokodaii
2.7.1.1 52
-
 CTP pH 7.5, 50°C Sulfurisphaera tokodaii
2.7.1.1 100
-
 N-acetyl-D-glucosamine pH 7.5, 50°C Sulfurisphaera tokodaii
2.7.1.1 170
-
 2-deoxy-D-glucose pH 7.5, 50°C Sulfurisphaera tokodaii
2.7.1.1 247
-
 D-glucosamine pH 7.5, 50°C Sulfurisphaera tokodaii
2.7.1.1 259
-
 ATP pH 7.5, 50°C Sulfurisphaera tokodaii
2.7.1.1 420
-
 D-mannose pH 7.5, 50°C Sulfurisphaera tokodaii
2.7.1.1 717
-
 D-glucose pH 7.5, 50°C Sulfurisphaera tokodaii