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Literature summary extracted from

  • Saxena, R.; Chakraborti, P.K.
    Identification of regions involved in enzymatic stability of peptide deformylase of Mycobacterium tuberculosis (2005), J. Bacteriol., 187, 8216-8220.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.88 into pET28c vector for over-expression in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.88 DELTA182-197 deletion mutant, amino acids 182-197 were removed from the carboxy-terminal end, inactive Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.88 cytoplasm
-
 Mycobacterium tuberculosis 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.88 Fe2+
-
 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.88 22600
-
 calculated molecular mass Mycobacterium tuberculosis
3.5.1.88 31000
-
 SDS-PAGE, Western blotting using an anti-his tag antibody Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.88 formyl-L-methionyl peptide + H2O Mycobacterium tuberculosis
-
 formate + methionyl peptide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.88 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.88 on Ni-NTA column Mycobacterium tuberculosis

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.5.1.88 solubilized with 3 M urea Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.88 formyl-L-methionyl peptide + H2O
-
 Mycobacterium tuberculosis formate + methionyl peptide
-
 ?
3.5.1.88 N-formylmethionine-alanine + H2O
-
 Mycobacterium tuberculosis formate + methionyl-alanine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.88 multimer mPDF in solution shows a molecular mass of 100 kDa, several mixed fractions of different mutants display enzyme activity Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
3.5.1.88 mPDF
-
 Mycobacterium tuberculosis
3.5.1.88 peptide deformylase
-
 Mycobacterium tuberculosis