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Literature summary extracted from

  • Seah, S.Y.; Labbe, G.; Kaschabek, S.R.; Reifenrath, F.; Reineke, W.; Eltis, L.D.
    Comparative specificities of two evolutionarily divergent hydrolases involved in microbial degradation of polychlorinated biphenyls (2001), J. Bacteriol., 183, 1511-1516.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.7.1.8 degradation key determinant in the aerobic transformation of polychlorinated biphenyls by divergent biphenyl degraders Rhodococcus globerulus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.8 overexpression in Pseudomonas putida KT2442 containing vector pSS316 Rhodococcus globerulus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.7.1.8 (2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid competetively inhibits the enzyme more effectively than (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid Rhodococcus globerulus
3.7.1.8 (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Rhodococcus globerulus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.8 0.00043
-
 (2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
 Rhodococcus globerulus
3.7.1.8 0.00047
-
 (2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid not chlorinated Rhodococcus globerulus
3.7.1.8 0.00065
-
 (2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid
-
 Rhodococcus globerulus
3.7.1.8 0.00075
-
 (2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
 Rhodococcus globerulus
3.7.1.8 0.00104
-
 (2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
 Rhodococcus globerulus
3.7.1.8 0.004
-
 (2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Rhodococcus globerulus
3.7.1.8 0.0044
-
 (2E,4Z)-2,4-dihydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Rhodococcus globerulus
3.7.1.8 0.0069
-
 (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Rhodococcus globerulus
3.7.1.8 0.0129
-
 (2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
 Rhodococcus globerulus
3.7.1.8 0.018
-
 (2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Rhodococcus globerulus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.8 32580
-
 by HPLC-ESMS analysis Rhodococcus globerulus

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.8 Rhodococcus globerulus
-
-
-
3.7.1.8 Rhodococcus globerulus P6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.8 to more than 99% homogeneity Rhodococcus globerulus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7.1.8 13.2
-
 purified enzyme Rhodococcus globerulus

Storage Stability

EC Number Storage Stability Organism
3.7.1.8 -80°C, 12 months Rhodococcus globerulus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.8 (2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus 4-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
3.7.1.8 (2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus P6 4-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
3.7.1.8 (2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus benzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
3.7.1.8 (2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus P6 benzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
3.7.1.8 (2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus 2-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
3.7.1.8 (2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus P6 2-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
3.7.1.8 (2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus 3,4-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
3.7.1.8 (2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus P6 3,4-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
3.7.1.8 (2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus 3,5-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
3.7.1.8 (2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus P6 3,5-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
3.7.1.8 (2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus 3-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
3.7.1.8 (2E,4Z)-2,4-dihydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus benzoate + (2E)-2,4-dihydroxypenta-2,4-dienoic acid
-
 ?
3.7.1.8 (2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus benzoate + (2E)-4-chloro-2-hydroxypenta-2,4-dienoic acid
-
 ?
3.7.1.8 (2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus benzoate + (2E,4Z)-5-chloro-2-hydroxypenta-2,4-dienoic acid
-
 ?
3.7.1.8 (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Rhodococcus globerulus benzoate + (2Z)-3-chloro-2-hydroxypenta-2,4-dienoic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.7.1.8 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase
-
 Rhodococcus globerulus
3.7.1.8 BphDP6
-
 Rhodococcus globerulus
3.7.1.8 HOPDA hydrolase
-
 Rhodococcus globerulus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7.1.8 0.0172
-
 (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Rhodococcus globerulus
3.7.1.8 1.03
-
 (2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
 Rhodococcus globerulus
3.7.1.8 1.74
-
 (2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
 Rhodococcus globerulus
3.7.1.8 7.6
-
 (2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid not chlorinated Rhodococcus globerulus
3.7.1.8 11.2
-
 (2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
 Rhodococcus globerulus
3.7.1.8 15.3
-
 (2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
 Rhodococcus globerulus
3.7.1.8 29
-
 (2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
 Rhodococcus globerulus
3.7.1.8 33.7
-
 (2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid
-
 Rhodococcus globerulus