BRENDA - Enzyme Database

Comparative specificities of two evolutionarily divergent hydrolases involved in microbial degradation of polychlorinated biphenyls

Seah, S.Y.; Labbe, G.; Kaschabek, S.R.; Reifenrath, F.; Reineke, W.; Eltis, L.D.; J. Bacteriol. 183, 1511-1516 (2001) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
3.7.1.8
degradation
key determinant in the aerobic transformation of polychlorinated biphenyls by divergent biphenyl degraders
Rhodococcus globerulus
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
3.7.1.8
overexpression in Pseudomonas putida KT2442 containing vector pSS316
Rhodococcus globerulus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.7.1.8
(2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
competetively inhibits the enzyme more effectively than (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
Rhodococcus globerulus
3.7.1.8
(2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.7.1.8
0.00043
-
(2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
0.00047
-
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
not chlorinated
Rhodococcus globerulus
3.7.1.8
0.00065
-
(2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
0.00075
-
(2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
0.00104
-
(2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
0.004
-
(2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
0.0044
-
(2E,4Z)-2,4-dihydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
0.0069
-
(2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
0.0129
-
(2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
0.018
-
(2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.7.1.8
32580
-
by HPLC-ESMS analysis
Rhodococcus globerulus
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.7.1.8
Rhodococcus globerulus
-
-
-
3.7.1.8
Rhodococcus globerulus P6
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.7.1.8
to more than 99% homogeneity
Rhodococcus globerulus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.7.1.8
13.2
-
purified enzyme
Rhodococcus globerulus
Storage Stability
EC Number
Storage Stability
Organism
3.7.1.8
-80°C, 12 months
Rhodococcus globerulus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.7.1.8
(2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
4-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus P6
4-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
benzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus P6
benzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
2-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus P6
2-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
3,4-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus P6
3,4-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
3,5-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus P6
3,5-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
3-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4Z)-2,4-dihydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
benzoate + (2E)-2,4-dihydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
benzoate + (2E)-4-chloro-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
benzoate + (2E,4Z)-5-chloro-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
benzoate + (2Z)-3-chloro-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
3.7.1.8
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase
-
Rhodococcus globerulus
3.7.1.8
BphDP6
-
Rhodococcus globerulus
3.7.1.8
HOPDA hydrolase
-
Rhodococcus globerulus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.7.1.8
0.0172
-
(2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
1.03
-
(2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
1.74
-
(2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
7.6
-
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
not chlorinated
Rhodococcus globerulus
3.7.1.8
11.2
-
(2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
15.3
-
(2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
29
-
(2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
33.7
-
(2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
Application (protein specific)
EC Number
Application
Commentary
Organism
3.7.1.8
degradation
key determinant in the aerobic transformation of polychlorinated biphenyls by divergent biphenyl degraders
Rhodococcus globerulus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.7.1.8
overexpression in Pseudomonas putida KT2442 containing vector pSS316
Rhodococcus globerulus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.7.1.8
(2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
competetively inhibits the enzyme more effectively than (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
Rhodococcus globerulus
3.7.1.8
(2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.7.1.8
0.00043
-
(2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
0.00047
-
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
not chlorinated
Rhodococcus globerulus
3.7.1.8
0.00065
-
(2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
0.00075
-
(2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
0.00104
-
(2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
0.004
-
(2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
0.0044
-
(2E,4Z)-2,4-dihydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
0.0069
-
(2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
0.0129
-
(2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
0.018
-
(2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.7.1.8
32580
-
by HPLC-ESMS analysis
Rhodococcus globerulus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.7.1.8
to more than 99% homogeneity
Rhodococcus globerulus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.7.1.8
13.2
-
purified enzyme
Rhodococcus globerulus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.7.1.8
-80°C, 12 months
Rhodococcus globerulus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.7.1.8
(2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
4-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus P6
4-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
benzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus P6
benzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
2-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus P6
2-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
3,4-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus P6
3,4-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
3,5-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus P6
3,5-dichlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
3-chlorobenzoate + (2E)-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4Z)-2,4-dihydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
benzoate + (2E)-2,4-dihydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4Z)-4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
benzoate + (2E)-4-chloro-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
benzoate + (2E,4Z)-5-chloro-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
3.7.1.8
(2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
674232
Rhodococcus globerulus
benzoate + (2Z)-3-chloro-2-hydroxypenta-2,4-dienoic acid
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.7.1.8
0.0172
-
(2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
1.03
-
(2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
1.74
-
(2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
7.6
-
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
not chlorinated
Rhodococcus globerulus
3.7.1.8
11.2
-
(2E,4E)-6-(3,5-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
15.3
-
(2E,4E)-6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
29
-
(2E,4E)-6-(3,4-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus
3.7.1.8
33.7
-
(2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid
-
Rhodococcus globerulus