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Literature summary extracted from

  • Sandrini, M.P.; Clausen, A.R.; On, S.L.; Aarestrup, F.M.; Munch-Petersen, B.; Piskur, J.
    Nucleoside analogues are activated by bacterial deoxyribonucleoside kinases in a species-specific manner (2007), J. Antimicrob. Chemother., 60, 510-520.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.76 expressed in Escherichia coli Bacillus cereus
2.7.1.76 expression in Escherichia coli Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.76 0.027
-
 2'-deoxycytidine
-
 Bacillus cereus
2.7.1.76 0.027
-
 deoxycytidine in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS Bacillus cereus
2.7.1.76 0.0332
-
 2'-deoxyadenosine
-
 Bacillus cereus
2.7.1.76 0.0332
-
 deoxyadenosine in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS Bacillus cereus
2.7.1.76 0.0335
-
 gemcitabine in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS Bacillus cereus
2.7.1.76 0.0335
-
 2',2'-difluorodeoxycytidine ratio kcat/Km is 5100 per s and M Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.76 26200
-
 SDS-PAGE Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.76 Bacillus cereus Q0H0H5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.76 glutathione Sepharose FF column chromatography Bacillus cereus
2.7.1.76 recombinant protein Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.76 ATP + 2',2'-difluorodeoxycytidine enzyme activates 2',2'-difluorodesoxycytidine in vivo Bacillus cereus ADP + 2',2'-difluorodeoxycytidine 5'-phosphate
-
 ?
2.7.1.76 ATP + 2'-deoxyadenosine
-
 Bacillus cereus ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.76 ATP + 2'-deoxycytidine
-
 Bacillus cereus ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.76 ATP + deoxyadenosine
-
 Bacillus cereus ADP + dAMP
-
 ?
2.7.1.76 ATP + deoxycytidine
-
 Bacillus cereus ADP + dCMP
-
 ?
2.7.1.76 ATP + gemcitabine
-
 Bacillus cereus ADP + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.76 dAK
-
 Bacillus cereus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.76 0.17
-
 gemcitabine in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS Bacillus cereus
2.7.1.76 0.55
-
 deoxycytidine in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS Bacillus cereus
2.7.1.76 1.22
-
 deoxyadenosine in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS Bacillus cereus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.76 ATP
-
 Bacillus cereus