BRENDA - Enzyme Database show

Nucleoside analogues are activated by bacterial deoxyribonucleoside kinases in a species-specific manner

Sandrini, M.P.; Clausen, A.R.; On, S.L.; Aarestrup, F.M.; Munch-Petersen, B.; Piskur, J.; J. Antimicrob. Chemother. 60, 510-520 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.76
expressed in Escherichia coli; expression in Escherichia coli
Bacillus cereus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.76
0.027
-
2'-deoxycytidine
-
Bacillus cereus
2.7.1.76
0.027
-
deoxycytidine
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
Bacillus cereus
2.7.1.76
0.0332
-
2'-deoxyadenosine
-
Bacillus cereus
2.7.1.76
0.0332
-
deoxyadenosine
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
Bacillus cereus
2.7.1.76
0.0335
-
2',2'-difluorodeoxycytidine
ratio kcat/Km is 5100 per s and M
Bacillus cereus
2.7.1.76
0.0335
-
gemcitabine
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
Bacillus cereus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.76
26200
-
SDS-PAGE
Bacillus cereus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.76
Bacillus cereus
Q0H0H5
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.76
glutathione Sepharose FF column chromatography; recombinant protein
Bacillus cereus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.76
ATP + 2',2'-difluorodeoxycytidine
enzyme activates 2',2'-difluorodesoxycytidine in vivo
674219
Bacillus cereus
ADP + 2',2'-difluorodeoxycytidine 5'-phosphate
-
-
-
?
2.7.1.76
ATP + 2'-deoxyadenosine
-
674219
Bacillus cereus
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.76
ATP + 2'-deoxycytidine
-
674219
Bacillus cereus
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.76
ATP + deoxyadenosine
-
674219
Bacillus cereus
ADP + dAMP
-
-
-
?
2.7.1.76
ATP + deoxycytidine
-
674219
Bacillus cereus
ADP + dCMP
-
-
-
?
2.7.1.76
ATP + gemcitabine
-
674219
Bacillus cereus
ADP + ?
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.76
0.17
-
gemcitabine
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
Bacillus cereus
2.7.1.76
0.55
-
deoxycytidine
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
Bacillus cereus
2.7.1.76
1.22
-
deoxyadenosine
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
Bacillus cereus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.76
ATP
-
Bacillus cereus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.76
expressed in Escherichia coli; expression in Escherichia coli
Bacillus cereus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.76
ATP
-
Bacillus cereus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.76
0.027
-
2'-deoxycytidine
-
Bacillus cereus
2.7.1.76
0.027
-
deoxycytidine
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
Bacillus cereus
2.7.1.76
0.0332
-
2'-deoxyadenosine
-
Bacillus cereus
2.7.1.76
0.0332
-
deoxyadenosine
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
Bacillus cereus
2.7.1.76
0.0335
-
2',2'-difluorodeoxycytidine
ratio kcat/Km is 5100 per s and M
Bacillus cereus
2.7.1.76
0.0335
-
gemcitabine
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
Bacillus cereus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.76
26200
-
SDS-PAGE
Bacillus cereus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.76
glutathione Sepharose FF column chromatography; recombinant protein
Bacillus cereus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.76
ATP + 2',2'-difluorodeoxycytidine
enzyme activates 2',2'-difluorodesoxycytidine in vivo
674219
Bacillus cereus
ADP + 2',2'-difluorodeoxycytidine 5'-phosphate
-
-
-
?
2.7.1.76
ATP + 2'-deoxyadenosine
-
674219
Bacillus cereus
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.76
ATP + 2'-deoxycytidine
-
674219
Bacillus cereus
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.76
ATP + deoxyadenosine
-
674219
Bacillus cereus
ADP + dAMP
-
-
-
?
2.7.1.76
ATP + deoxycytidine
-
674219
Bacillus cereus
ADP + dCMP
-
-
-
?
2.7.1.76
ATP + gemcitabine
-
674219
Bacillus cereus
ADP + ?
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.76
0.17
-
gemcitabine
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
Bacillus cereus
2.7.1.76
0.55
-
deoxycytidine
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
Bacillus cereus
2.7.1.76
1.22
-
deoxyadenosine
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
Bacillus cereus