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Literature summary extracted from

  • Peng, C.C.; Hsiao, E.S.; Ding, J.L.; Tzen, J.T.
    Functional expression in pichia pastoris of an acidic pectin methylesterase from jelly fig (Ficus awkeotsang) (2005), J. Agric. Food Chem., 53, 5612-5616.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.11 expressed in Pichia pastoris as secretory protein Ficus pumila var. awkeotsang

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.11 38000
-
x * 38000, SDS-PAGE, protein from host Ficus pumila var. awkeotsang
3.1.1.11 45000
-
x * 45000, SDS-PAGE, recombinant protein Ficus pumila var. awkeotsang

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.11 pectin + H2O Ficus pumila var. awkeotsang involved in the regulation of the cell wall rigidity, key role in process of fruit ripening methanol + pectate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.11 Ficus pumila var. awkeotsang
-
jelly fig Makino
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.11 glycoprotein native protein has about 10% carbohydrates, recombinant protein appears to be hyperglycosylated Ficus pumila var. awkeotsang

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.11 recombinant protein and native protein from host Ficus pumila var. awkeotsang

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.11 achene
-
Ficus pumila var. awkeotsang
-

Storage Stability

EC Number Storage Stability Organism
3.1.1.11 4°C, fully stable for several months Ficus pumila var. awkeotsang
3.1.1.11 room temperature, fully stable for several days Ficus pumila var. awkeotsang

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.11 pectin + H2O
-
Ficus pumila var. awkeotsang methanol + pectate
-
?
3.1.1.11 pectin + H2O involved in the regulation of the cell wall rigidity, key role in process of fruit ripening Ficus pumila var. awkeotsang methanol + pectate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.11 ? x * 38000, SDS-PAGE, protein from host Ficus pumila var. awkeotsang
3.1.1.11 ? x * 45000, SDS-PAGE, recombinant protein Ficus pumila var. awkeotsang

Synonyms

EC Number Synonyms Comment Organism
3.1.1.11 pectin methylesterase
-
Ficus pumila var. awkeotsang
3.1.1.11 PME
-
Ficus pumila var. awkeotsang

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.11 50
-
no loss of activity within 2 h Ficus pumila var. awkeotsang
3.1.1.11 65
-
native enzyme stable for 30 min, 60% of activity remaining after 2 h Ficus pumila var. awkeotsang
3.1.1.11 70
-
20% of activity remaining after 2 h Ficus pumila var. awkeotsang

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.11 6
-
native and recombinant protein Ficus pumila var. awkeotsang

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.11 3 9
-
Ficus pumila var. awkeotsang