EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.11 | expressed in Pichia pastoris as secretory protein | Ficus pumila var. awkeotsang |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.11 | 38000 | - |
x * 38000, SDS-PAGE, protein from host | Ficus pumila var. awkeotsang |
3.1.1.11 | 45000 | - |
x * 45000, SDS-PAGE, recombinant protein | Ficus pumila var. awkeotsang |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.11 | pectin + H2O | Ficus pumila var. awkeotsang | involved in the regulation of the cell wall rigidity, key role in process of fruit ripening | methanol + pectate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.11 | Ficus pumila var. awkeotsang | - |
jelly fig Makino | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.1.11 | glycoprotein | native protein has about 10% carbohydrates, recombinant protein appears to be hyperglycosylated | Ficus pumila var. awkeotsang |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.11 | recombinant protein and native protein from host | Ficus pumila var. awkeotsang |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.11 | achene | - |
Ficus pumila var. awkeotsang | - |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.1.11 | 4°C, fully stable for several months | Ficus pumila var. awkeotsang |
3.1.1.11 | room temperature, fully stable for several days | Ficus pumila var. awkeotsang |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.11 | pectin + H2O | - |
Ficus pumila var. awkeotsang | methanol + pectate | - |
? | |
3.1.1.11 | pectin + H2O | involved in the regulation of the cell wall rigidity, key role in process of fruit ripening | Ficus pumila var. awkeotsang | methanol + pectate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.11 | ? | x * 38000, SDS-PAGE, protein from host | Ficus pumila var. awkeotsang |
3.1.1.11 | ? | x * 45000, SDS-PAGE, recombinant protein | Ficus pumila var. awkeotsang |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.11 | pectin methylesterase | - |
Ficus pumila var. awkeotsang |
3.1.1.11 | PME | - |
Ficus pumila var. awkeotsang |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.11 | 50 | - |
no loss of activity within 2 h | Ficus pumila var. awkeotsang |
3.1.1.11 | 65 | - |
native enzyme stable for 30 min, 60% of activity remaining after 2 h | Ficus pumila var. awkeotsang |
3.1.1.11 | 70 | - |
20% of activity remaining after 2 h | Ficus pumila var. awkeotsang |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.11 | 6 | - |
native and recombinant protein | Ficus pumila var. awkeotsang |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.11 | 3 | 9 | - |
Ficus pumila var. awkeotsang |