BRENDA - Enzyme Database

Eimeria tenella enolase and pyruvate kinase: a likely role in glycolysis and in others functions

Labbe, M.; Peroval, M.; Bourdieu, C.; Girard-Misguich, F.; Pery, P.; Int. J. Parasitol. 36, 1443-1452 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.40
expressed in Escherichia coli
Eimeria tenella
4.2.1.11
identified from cDNA library, expression in Escherichia coli
Eimeria tenella
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.40
nucleus
in the nucleus of schizonts and sporozoites
Eimeria tenella
5634
-
4.2.1.11
cytoplasm
partly localized inside the nucleus of sporozoites and schizonts, partly secreted, apex of the first generation of merozoites, relocalization inside sporozoites observed
Eimeria tenella
5737
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.40
55000
-
SDS-PAGE
Eimeria tenella
2.7.1.40
57590
-
calculated from amino acid sequence
Eimeria tenella
4.2.1.11
50000
-
gel electrophoresis
Eimeria tenella
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Eimeria tenella
-
phosphoenolpyruvate + H2O
-
-
r
4.2.1.11
2-phospho-D-glycerate
Eimeria tenella PaPt36
-
phosphoenolpyruvate + H2O
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.40
Eimeria tenella
O44006
-
-
2.7.1.40
Eimeria tenella PaPt36
O44006
-
-
4.2.1.11
Eimeria tenella
Q967Y8
-
-
4.2.1.11
Eimeria tenella PaPt36
Q967Y8
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.40
Ni-Sepharose column chromatography
Eimeria tenella
4.2.1.11
recombinant protein, pET22/14-X vector, His-tag purification, SDS-PAGE
Eimeria tenella
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.40
merozoite
-
Eimeria tenella
-
2.7.1.40
schizont
-
Eimeria tenella
-
2.7.1.40
sporozoite
-
Eimeria tenella
-
4.2.1.11
schizont
isolated after infection of 2-week-old White Leghorn PA12 chickens for 68 h, protein but not cDNA detected in the sporozoite stage, expression enhanced during the first schizogony
Eimeria tenella
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
multifunctional role of enolase, participation in the parasitic invasion process and in the control of gene regulation
Eimeria tenella
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
674072
Eimeria tenella
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
674072
Eimeria tenella PaPt36
phosphoenolpyruvate + H2O
-
-
-
r
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.40
expressed in Escherichia coli
Eimeria tenella
4.2.1.11
identified from cDNA library, expression in Escherichia coli
Eimeria tenella
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.40
nucleus
in the nucleus of schizonts and sporozoites
Eimeria tenella
5634
-
4.2.1.11
cytoplasm
partly localized inside the nucleus of sporozoites and schizonts, partly secreted, apex of the first generation of merozoites, relocalization inside sporozoites observed
Eimeria tenella
5737
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.40
55000
-
SDS-PAGE
Eimeria tenella
2.7.1.40
57590
-
calculated from amino acid sequence
Eimeria tenella
4.2.1.11
50000
-
gel electrophoresis
Eimeria tenella
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Eimeria tenella
-
phosphoenolpyruvate + H2O
-
-
r
4.2.1.11
2-phospho-D-glycerate
Eimeria tenella PaPt36
-
phosphoenolpyruvate + H2O
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.40
Ni-Sepharose column chromatography
Eimeria tenella
4.2.1.11
recombinant protein, pET22/14-X vector, His-tag purification, SDS-PAGE
Eimeria tenella
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.40
merozoite
-
Eimeria tenella
-
2.7.1.40
schizont
-
Eimeria tenella
-
2.7.1.40
sporozoite
-
Eimeria tenella
-
4.2.1.11
schizont
isolated after infection of 2-week-old White Leghorn PA12 chickens for 68 h, protein but not cDNA detected in the sporozoite stage, expression enhanced during the first schizogony
Eimeria tenella
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
multifunctional role of enolase, participation in the parasitic invasion process and in the control of gene regulation
Eimeria tenella
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
674072
Eimeria tenella
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
674072
Eimeria tenella PaPt36
phosphoenolpyruvate + H2O
-
-
-
r