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Literature summary extracted from

  • Xia, Y.; Park, Y.D.; Mu, H.; Zhou, H.M.; Wang, X.Y.; Meng, F.G.
    The protective effects of osmolytes on arginine kinase unfolding and aggregation (2007), Int. J. Biol. Macromol., 40, 437-443.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.3.3 DMSO protects arginine kinase from inactivation losing its native tertiary conformation and aggregation in the presence of guanidine hydrochloride Locusta migratoria manilensis
2.7.3.3 glycerol protects arginine kinase from inactivation in both low and high concentrations of guanidine hydrochloride Locusta migratoria manilensis
2.7.3.3 proline with proline the residual activity of AK after incubation in guanidine hydrochloride is higher than without proline Locusta migratoria manilensis
2.7.3.3 sucrose maintains the activity of the enzyme in the presence of guanidine hydrochloride Locusta migratoria manilensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.3.3 guanidine hydrochloride 1 mM Locusta migratoria manilensis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.7.3.3 DMSO prevents arginine kinase unfolding and inactivation that is due to a guanidine hydrochloride denaturant by reducing the inactivation rate constants, increasing the transition free energy changes and increasing the value for the midpoint of denaturation Locusta migratoria manilensis
2.7.3.3 Glycerol prevents arginine kinase unfolding and inactivation that is due to a guanidine hydrochloride denaturant by reducing the inactivation rate constants, increasing the transition free energy changes and increasing the value for the midpoint of denaturation Locusta migratoria manilensis
2.7.3.3 guanidine-HCl 2.0 M guanidine hydrochloride results in almost complete unfolding of arginine kinase. When adding the osmolytes (Pro, sucrose, DMSO, glycerol) to the unfolding system, 2.0 M guanidine hydrochloride only brings about a partial red shift compared to the samples without osmolyte. Osmolytes stabilize the arginine kinase conformation against guanidine hydrochloride in a concentration-dependent manner Locusta migratoria manilensis

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.3 Locusta migratoria manilensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.3
-
 Locusta migratoria manilensis
2.7.3.3 Sephacryl S-200HR gel filtration and DEAE Sepharose CL-6B fast flow chromatography Locusta migratoria manilensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.3 muscle
-
 Locusta migratoria manilensis
-
2.7.3.3 muscle leg muscle Locusta migratoria manilensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.3 ATP + L-Arg
-
 Locusta migratoria manilensis ADP + Nomega-phospho-L-Arg
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.3.3 ATP:L-arginine phosphotransferase
-
 Locusta migratoria manilensis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.3.3 ATP
-
 Locusta migratoria manilensis