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Literature summary extracted from

  • Zhang, J.W.; Zhao, T.J.; Wang, S.L.; Guo, Q.; Liu, T.T.; Zhao, F.; Wang, X.C.
    The roles of C-terminal loop residues of dimeric arginine kinase from sea cucumber Stichopus japonicus in catalysis, specificity and structure (2006), Int. J. Biol. Macromol., 38, 203-210.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.3 expressed in Escherichia coli BL21 (DE3) cells Apostichopus japonicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.3.3 E314D significantly decreased activity Apostichopus japonicus
2.7.3.3 E314Q significantly decreased activity Apostichopus japonicus
2.7.3.3 E314V significantly decreased activity Apostichopus japonicus
2.7.3.3 F315A modest AK activity Apostichopus japonicus
2.7.3.3 F315H modest AK activity Apostichopus japonicus
2.7.3.3 F315Y modest AK activity Apostichopus japonicus
2.7.3.3 S312G/E314V/F315D/E317A/S318A/G321S slight arginine kinase activity Apostichopus japonicus
2.7.3.3 S312R/F315H/V319E modest AK activity Apostichopus japonicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.3 0.52
-
 L-Arg wild type enzyme Apostichopus japonicus
2.7.3.3 0.84
-
 L-Arg mutant enzyme F315Y Apostichopus japonicus
2.7.3.3 0.99
-
 L-Arg mutant enzyme S312R/F315H/V319E Apostichopus japonicus
2.7.3.3 1.04
-
 ATP wild type enzyme Apostichopus japonicus
2.7.3.3 1.13
-
 L-Arg mutant enzyme F315H Apostichopus japonicus
2.7.3.3 1.49
-
 ATP mutant enzyme F315Y Apostichopus japonicus
2.7.3.3 1.49
-
 ATP mutant enzyme S312R/F315H/V319E Apostichopus japonicus
2.7.3.3 2.08
-
 ATP mutant enzyme F315H Apostichopus japonicus
2.7.3.3 2.67
-
 L-Arg mutant enzyme F315A Apostichopus japonicus
2.7.3.3 2.76
-
 ATP mutant enzyme F315A Apostichopus japonicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.3 Apostichopus japonicus
-
 sea cucumber
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.3 Ni2+-nitrilotriacetic acid column chromatography and Superdex 200HR 10/30 gel filtration Apostichopus japonicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.3 ATP + L-Arg
-
 Apostichopus japonicus ADP + Nomega-phospho-L-Arg
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.3.3 dimer
-
 Apostichopus japonicus

Synonyms

EC Number Synonyms Comment Organism
2.7.3.3 ATP: arginine N-phosphotransferase
-
 Apostichopus japonicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.3.3 0.08
-
 L-Arg mutant enzyme S312G/E314V/F315D/E317A/S318A/G321S Apostichopus japonicus
2.7.3.3 0.833
-
 L-Arg mutant enzyme F315Y Apostichopus japonicus
2.7.3.3 1.02
-
 L-Arg mutant enzyme F315A Apostichopus japonicus
2.7.3.3 1.1
-
 L-Arg wild type enzyme Apostichopus japonicus
2.7.3.3 3.97
-
 L-Arg mutant enzyme F315H Apostichopus japonicus
2.7.3.3 5.54
-
 L-Arg mutant enzyme S312R/F315H/V319E Apostichopus japonicus
2.7.3.3 15.67
-
 L-Arg mutant enzyme F315Y Apostichopus japonicus
2.7.3.3 18.66
-
 L-Arg wild type enzyme Apostichopus japonicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.3.3 ATP
-
 Apostichopus japonicus