BRENDA - Enzyme Database

Purification, identification and characterisation of seprase from bovine serum

Collins, P.J.; McMahon, G.; OBrien, P.; OConnor, B.; Int. J. Biochem. Cell Biol. 36, 2320-2333 (2004) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.21.B28
diisopropylfluorophosphate
irreversible inhibition
Bos taurus
3.4.21.B28
additional information
not inhibited by JTP-4819, Fmoc-Ala-pyrrCN and N-benzyloxycarbonyl-Phe-Pro-BT
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.21.B28
0.27
-
Z-Gly-Pro-4-methylcoumarin-7-amide
-
Bos taurus
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.4.21.B28
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.4.21.B28
by column chromatographies, 30197fold to homogeneity
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.21.B28
blood serum
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.4.21.B28
Gelatin + H2O
-
674022
Bos taurus
?
-
-
-
?
3.4.21.B28
additional information
no hydrolysis of Pro-7-amino-4-methylcoumarin, Gly-Pro-7-amino-4-methylcoumarin, Ala-Pro-7-amino-4-methylcoumarin, pGly-His-Pro-7-amino-4-methylcoumarin, N-benzyloxycarbonyl-Pro-Phe, Gly-Gly-Pro-Ala and N-benzyloxycarbonyl-Gly-Leu-Phe-His
674022
Bos taurus
?
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Leu-Phe-His + H2O
-
674022
Bos taurus
?
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + 7-amino-4-methylcoumarin
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Ala + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + L-Ala
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Glu + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + L-Glu
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-His + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + L-His
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Leu + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + L-Leu
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Met + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + L-Met
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Met-His + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + Met-His
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Met-His-Arg-Ser + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + Met-His-Arg-Ser
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Phe + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + L-Phe
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Phe-His + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + L-Phe-L-His
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Phe-His-Arg + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + Phe-His-Arg
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Phe-His-Arg-Ser + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + Phe-His-Arg-Ser
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Ser + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + L-Ser
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Tyr + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + L-Tyr
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
3.4.21.B28
fibroblast activation protein alpha
-
Bos taurus
3.4.21.B28
seprase
-
Bos taurus
3.4.21.B28
Z-Pro-prolinal-insensitive peptidase
-
Bos taurus
3.4.21.B28
ZIP
-
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.21.B28
diisopropylfluorophosphate
irreversible inhibition
Bos taurus
3.4.21.B28
additional information
not inhibited by JTP-4819, Fmoc-Ala-pyrrCN and N-benzyloxycarbonyl-Phe-Pro-BT
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.21.B28
0.27
-
Z-Gly-Pro-4-methylcoumarin-7-amide
-
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.21.B28
by column chromatographies, 30197fold to homogeneity
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.21.B28
blood serum
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.4.21.B28
Gelatin + H2O
-
674022
Bos taurus
?
-
-
-
?
3.4.21.B28
additional information
no hydrolysis of Pro-7-amino-4-methylcoumarin, Gly-Pro-7-amino-4-methylcoumarin, Ala-Pro-7-amino-4-methylcoumarin, pGly-His-Pro-7-amino-4-methylcoumarin, N-benzyloxycarbonyl-Pro-Phe, Gly-Gly-Pro-Ala and N-benzyloxycarbonyl-Gly-Leu-Phe-His
674022
Bos taurus
?
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Leu-Phe-His + H2O
-
674022
Bos taurus
?
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + 7-amino-4-methylcoumarin
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Ala + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + L-Ala
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Glu + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + L-Glu
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-His + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + L-His
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Leu + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + L-Leu
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Met + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + L-Met
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Met-His + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + Met-His
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Met-His-Arg-Ser + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + Met-His-Arg-Ser
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Phe + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + L-Phe
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Phe-His + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + L-Phe-L-His
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Phe-His-Arg + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + Phe-His-Arg
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Phe-His-Arg-Ser + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + Phe-His-Arg-Ser
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Ser + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + L-Ser
-
-
-
?
3.4.21.B28
N-benzyloxycarbonyl-Gly-Pro-Tyr + H2O
-
674022
Bos taurus
N-benzyloxycarbonyl-Gly-Pro + L-Tyr
-
-
-
?