BRENDA - Enzyme Database

Arginine kinase from the beetle Cissites cephalotes (Olivier). Molecular cloning, phylogenetic analysis and enzymatic properties

Tanaka, K.; Ichinari, S.; Iwanami, K.; Yoshimatsu, S.; Suzuki, T.; Insect Biochem. Mol. Biol. 37, 338-345 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.3.3
expressed in Escherichia coli as a fusion with maltose-binding protein; expression of Cissites arginine kinase protein in Escherichia coli as a fusion with maltose-binding protein
Cissites cephalotes
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.3.3
0.95
-
ATP
in 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, at 25°C; pH 8, 25°C
Cissites cephalotes
2.7.3.3
1.01
-
L-Arg
in 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, at 25°C; pH 8, 25°C
Cissites cephalotes
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.3
40000
-
SDS-PAGE
Cissites cephalotes
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.3.3
Cissites cephalotes
A4F2K7
-
-
2.7.3.3
Cissites cephalotes
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ADP + omega-N-phospho-L-Arg
function: five residues predicted to interact with the substrate arginine (S77, Y82, E239, C285 and E328), and five residues predicted to interact with the substrate ADP (R138, R140, R243, R294 and R323). Arginine (or phosphagen) and MgATP (or MgADP), typically exhibit synergistic binding to arginine kinase
674014
Cissites cephalotes
ATP + L-arginine
-
-
-
r
2.7.3.3
ATP + L-Arg
-
674014
Cissites cephalotes
ADP + Nomega-phospho-L-Arg
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.3.3
2.02
-
ATP
pH 8, 25°C
Cissites cephalotes
2.7.3.3
2.02
-
L-Arg
in 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, at 25°C; pH 8, 25°C
Cissites cephalotes
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.3.3
ATP
-
Cissites cephalotes
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.3.3
expressed in Escherichia coli as a fusion with maltose-binding protein
Cissites cephalotes
2.7.3.3
expression of Cissites arginine kinase protein in Escherichia coli as a fusion with maltose-binding protein
Cissites cephalotes
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.3.3
ATP
-
Cissites cephalotes
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.3.3
0.95
-
ATP
in 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, at 25°C
Cissites cephalotes
2.7.3.3
0.95
-
ATP
pH 8, 25°C
Cissites cephalotes
2.7.3.3
1.01
-
L-Arg
in 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, at 25°C
Cissites cephalotes
2.7.3.3
1.01
-
L-Arg
pH 8, 25°C
Cissites cephalotes
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.3
40000
-
SDS-PAGE
Cissites cephalotes
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ADP + omega-N-phospho-L-Arg
function: five residues predicted to interact with the substrate arginine (S77, Y82, E239, C285 and E328), and five residues predicted to interact with the substrate ADP (R138, R140, R243, R294 and R323). Arginine (or phosphagen) and MgATP (or MgADP), typically exhibit synergistic binding to arginine kinase
674014
Cissites cephalotes
ATP + L-arginine
-
-
-
r
2.7.3.3
ATP + L-Arg
-
674014
Cissites cephalotes
ADP + Nomega-phospho-L-Arg
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.3.3
2.02
-
ATP
pH 8, 25°C
Cissites cephalotes
2.7.3.3
2.02
-
L-Arg
in 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, at 25°C
Cissites cephalotes
2.7.3.3
2.02
-
L-Arg
pH 8, 25°C
Cissites cephalotes