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Literature summary extracted from

  • Tanaka, K.; Ichinari, S.; Iwanami, K.; Yoshimatsu, S.; Suzuki, T.
    Arginine kinase from the beetle Cissites cephalotes (Olivier). Molecular cloning, phylogenetic analysis and enzymatic properties (2007), Insect Biochem. Mol. Biol., 37, 338-345.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.3 expressed in Escherichia coli as a fusion with maltose-binding protein Cissites cephalotes
2.7.3.3 expression of Cissites arginine kinase protein in Escherichia coli as a fusion with maltose-binding protein Cissites cephalotes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.3 0.95
-
 ATP pH 8, 25°C Cissites cephalotes
2.7.3.3 0.95
-
 ATP in 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, at 25°C Cissites cephalotes
2.7.3.3 1.01
-
 L-Arg pH 8, 25°C Cissites cephalotes
2.7.3.3 1.01
-
 L-Arg in 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, at 25°C Cissites cephalotes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.3.3 40000
-
 SDS-PAGE Cissites cephalotes

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.3 Cissites cephalotes
-
-
-
2.7.3.3 Cissites cephalotes A4F2K7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.3 ADP + omega-N-phospho-L-Arg function: five residues predicted to interact with the substrate arginine (S77, Y82, E239, C285 and E328), and five residues predicted to interact with the substrate ADP (R138, R140, R243, R294 and R323). Arginine (or phosphagen) and MgATP (or MgADP), typically exhibit synergistic binding to arginine kinase Cissites cephalotes ATP + L-arginine
-
 r
2.7.3.3 ATP + L-Arg
-
 Cissites cephalotes ADP + Nomega-phospho-L-Arg
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.3.3 2.02
-
 ATP pH 8, 25°C Cissites cephalotes
2.7.3.3 2.02
-
 L-Arg pH 8, 25°C Cissites cephalotes
2.7.3.3 2.02
-
 L-Arg in 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, at 25°C Cissites cephalotes

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.3.3 ATP
-
 Cissites cephalotes