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Literature summary extracted from

  • Rudd, D.J.; Sazinsky, M.H.; Lippard, S.J.; Hedman, B.; Hodgson, K.O.
    X-ray absorption spectroscopic study of the reduced hydroxylases of methane monooxygenase and toluene/o-xylene monooxygenase: differences in active site structure and effects of the coupling proteins MMOB and ToMOD (2005), Inorg. Chem., 44, 4546-4554.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.25 crystal structure analysis Methylococcus capsulatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.25 Fe2+ MOOH contains 3.7-4.1 Fe atoms per dimer, binding structure and geometric configuration, EXAFS and Fourier transformation analysis, detailed overview Methylococcus capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.25 methane + NADH + O2 Methylococcus capsulatus
-
 methanol + NAD+ + H2O
-
 ?
1.14.13.25 methane + NADH + O2 Methylococcus capsulatus Bath
-
 methanol + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.25 Methylococcus capsulatus
-
-
-
1.14.13.25 Methylococcus capsulatus Bath
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.25 methane + NADH + O2
-
 Methylococcus capsulatus methanol + NAD+ + H2O
-
 ?
1.14.13.25 methane + NADH + O2
-
 Methylococcus capsulatus Bath methanol + NAD+ + H2O
-
 ?
1.14.13.25 propylene + NADH + O2
-
 Methylococcus capsulatus propylene epoxide + NAD+ + H2O
-
 ?
1.14.13.25 propylene + NADH + O2
-
 Methylococcus capsulatus Bath propylene epoxide + NAD+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.25 MMOH
-
 Methylococcus capsulatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.25 25
-
 assay at Methylococcus capsulatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.25 0.23
-
 propylene 25°C, purified enzyme Methylococcus capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.25 NADH
-
 Methylococcus capsulatus