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Literature summary extracted from

  • Uitto, V.
    Trepolisin (2004), Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , Eds. ) Academic Press, 2, 1813-1815.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.B21 cysteine increases activity Treponema denticola
3.4.21.B21 DTT increases activity Treponema denticola

Application

EC Number Application Comment Organism
3.4.21.B21 additional information contributes to tissue invasion and lysis of host cells, important in migration of the organism through basement membrane, cytopathic effects on epithelial cells, able to trigger release of polymorphonuclear leukocyte granules and activates latent matrix metalloproteinases Treponema denticola

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.B21 Bowman-Birk soybean trypsin-chymotrypsin inhibitor
-
 Treponema denticola
3.4.21.B21 DFP inactivates Treponema denticola
3.4.21.B21 diethyl dicarbonate inactivates Treponema denticola
3.4.21.B21 additional information not inhibited by Tos-Lys-CH2Cl, alpha1-proteinase inhibitor and pepstatin Treponema denticola
3.4.21.B21 N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline inactivates Treponema denticola
3.4.21.B21 PMSF
-
 Treponema denticola
3.4.21.B21 thiol-blocking reagent
-
 Treponema denticola
3.4.21.B21 Tos-Phe-CH2Cl
-
 Treponema denticola

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.B21 outer membrane
-
 Treponema denticola 19867
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.B21 Ca2+ increases activity somewhat or has no effect Treponema denticola

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.B21 95000
-
-
 Treponema denticola

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B21 Treponema denticola
-
 ATCC strains 33520 and 35405
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B21 rapidly purified by sonication, SDS-PAGE, gel filtration, pressure filtration, 101fold purified by ion-exchange chromatography followed by gel filtration Treponema denticola

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.4.21.B21 heating at 100°C results in formation of three polypeptides Treponema denticola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B21 alpha1-proteinase inhibitor + H2O
-
 Treponema denticola ?
-
 ?
3.4.21.B21 angiotensin + H2O
-
 Treponema denticola ?
-
 ?
3.4.21.B21 casein + H2O
-
 Treponema denticola ?
-
 ?
3.4.21.B21 Fibrinogen + H2O
-
 Treponema denticola ?
-
 ?
3.4.21.B21 Fibronectin + H2O
-
 Treponema denticola ?
-
 ?
3.4.21.B21 Gelatin + H2O
-
 Treponema denticola ?
-
 ?
3.4.21.B21 IgA + H2O
-
 Treponema denticola ?
-
 ?
3.4.21.B21 IgG + H2O
-
 Treponema denticola ?
-
 ?
3.4.21.B21 Laminin + H2O
-
 Treponema denticola ?
-
 ?
3.4.21.B21 additional information no degradation of native type I collagen Treponema denticola ?
-
 ?
3.4.21.B21 serum albumin + H2O
-
 Treponema denticola ?
-
 ?
3.4.21.B21 Substance P + H2O
-
 Treponema denticola ?
-
 ?
3.4.21.B21 Suc-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
-
 Treponema denticola Suc-Ala-Ala-Pro-Phe + p-nitroaniline
-
 ?
3.4.21.B21 transferrin + H2O
-
 Treponema denticola ?
-
 ?
3.4.21.B21 Type IV collagen + H2O
-
 Treponema denticola ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B21 chymotrypsin-like proteinase
-
 Treponema denticola
3.4.21.B21 dentilisin
-
 Treponema denticola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B21 7.5 8
-
 Treponema denticola