BRENDA - Enzyme Database

The crtS gene of Xanthophyllomyces dendrorhous encodes a novel cytochrome-P450 hydroxylase involved in the conversion of beta-carotene into astaxanthin and other xanthophylls

Alvarez, V.; Rodriguez-Saiz, M.; de la Fuente, J.L.; Gudina, E.J.; Godio, R.P.; Martin, J.F.; Barredo, J.L.; Fungal Genet. Biol. 43, 261-272 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.14.1
gene crtS, DNA and amino acid sequence determination of different strains
Xanthophyllomyces dendrorhous
1.14.15.24
expressed in Mucor circinelloides strain MS12
Xanthophyllomyces dendrorhous
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.14.14.1
additional information
construction and analysis of insertion mutant strains defective in biosynthesis of astaxanthin, the mutants are selected by accumulation of astaxanthin precursors
Xanthophyllomyces dendrorhous
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.14.1
Fe2+
heme iron
Xanthophyllomyces dendrorhous
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.15.24
62600
-
deduced from amino acid sequence
Xanthophyllomyces dendrorhous
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.1
additional information
Xanthophyllomyces dendrorhous
the enzyme is involved in the biosynthesis of astaxanthin, overview
?
-
-
-
1.14.14.1
additional information
Xanthophyllomyces dendrorhous VKPM Y2410
the enzyme is involved in the biosynthesis of astaxanthin, overview
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.1
Xanthophyllomyces dendrorhous
-
wild-type strains ATCC 24203, ATCC 96594, and ATCC 96815, and astaxanthin-overproducing recombinant strains VKPM and Y2410, gene crtS
-
1.14.15.24
Xanthophyllomyces dendrorhous
-
-
-
1.14.15.24
Xanthophyllomyces dendrorhous VKPM Y2410
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.1
additional information
the enzyme is involved in the biosynthesis of astaxanthin, overview
673817
Xanthophyllomyces dendrorhous
?
-
-
-
-
1.14.14.1
additional information
the enzyme is involved in the biosynthesis of astaxanthin, overview
673817
Xanthophyllomyces dendrorhous VKPM Y2410
?
-
-
-
-
1.14.15.24
3'-hydroxyechinenone + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
673817
Xanthophyllomyces dendrorhous
adonixanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
3'-hydroxyechinenone + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
673817
Xanthophyllomyces dendrorhous VKPM Y2410
adonixanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
3-hydroxyechinenone + NADH + H+ + O2
-
673817
Xanthophyllomyces dendrorhous
adonixanthin + NAD+ + H2O
-
-
-
?
1.14.15.24
3-hydroxyechinenone + NADH + H+ + O2
-
673817
Xanthophyllomyces dendrorhous VKPM Y2410
adonixanthin + NAD+ + H2O
-
-
-
?
1.14.15.24
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
673817
Xanthophyllomyces dendrorhous
beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
beta-cryptoxanthin is 3-hydroxy-beta,beta-carotene
-
-
?
1.14.15.24
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
673817
Xanthophyllomyces dendrorhous VKPM Y2410
beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
beta-cryptoxanthin is 3-hydroxy-beta,beta-carotene
-
-
?
1.14.15.24
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
673817
Xanthophyllomyces dendrorhous
zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
zeaxanthin is 3,3'-dihydroxy-beta,beta-carotene
-
-
?
1.14.15.24
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
673817
Xanthophyllomyces dendrorhous VKPM Y2410
zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
zeaxanthin is 3,3'-dihydroxy-beta,beta-carotene
-
-
?
1.14.15.24
beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
673817
Xanthophyllomyces dendrorhous
zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
canthaxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
canthaxanthin is beta,beta-carotene-4-4'-dione
673817
Xanthophyllomyces dendrorhous
phoenicoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
echinenone + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
echinenone is beta,beta-carotene-4-one
673817
Xanthophyllomyces dendrorhous
3-hydroxyechinenone + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
phoenicoxanthin + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
673817
Xanthophyllomyces dendrorhous
astaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
astaxanthin is 3,3'-dihydroxy-beta,beta-carotene-4-4'-dione
-
-
?
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.14.15.24
Xanthophyllomyces dendrorhous
deduced from amino acid sequence
-
5.8
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.1
gene crtS, DNA and amino acid sequence determination of different strains
Xanthophyllomyces dendrorhous
1.14.15.24
expressed in Mucor circinelloides strain MS12
Xanthophyllomyces dendrorhous
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.14.14.1
additional information
construction and analysis of insertion mutant strains defective in biosynthesis of astaxanthin, the mutants are selected by accumulation of astaxanthin precursors
Xanthophyllomyces dendrorhous
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.14.1
Fe2+
heme iron
Xanthophyllomyces dendrorhous
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.15.24
62600
-
deduced from amino acid sequence
Xanthophyllomyces dendrorhous
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.1
additional information
Xanthophyllomyces dendrorhous
the enzyme is involved in the biosynthesis of astaxanthin, overview
?
-
-
-
1.14.14.1
additional information
Xanthophyllomyces dendrorhous VKPM Y2410
the enzyme is involved in the biosynthesis of astaxanthin, overview
?
-
-
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.1
additional information
the enzyme is involved in the biosynthesis of astaxanthin, overview
673817
Xanthophyllomyces dendrorhous
?
-
-
-
-
1.14.14.1
additional information
the enzyme is involved in the biosynthesis of astaxanthin, overview
673817
Xanthophyllomyces dendrorhous VKPM Y2410
?
-
-
-
-
1.14.15.24
3'-hydroxyechinenone + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
673817
Xanthophyllomyces dendrorhous
adonixanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
3'-hydroxyechinenone + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
673817
Xanthophyllomyces dendrorhous VKPM Y2410
adonixanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
3-hydroxyechinenone + NADH + H+ + O2
-
673817
Xanthophyllomyces dendrorhous
adonixanthin + NAD+ + H2O
-
-
-
?
1.14.15.24
3-hydroxyechinenone + NADH + H+ + O2
-
673817
Xanthophyllomyces dendrorhous VKPM Y2410
adonixanthin + NAD+ + H2O
-
-
-
?
1.14.15.24
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
673817
Xanthophyllomyces dendrorhous
beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
beta-cryptoxanthin is 3-hydroxy-beta,beta-carotene
-
-
?
1.14.15.24
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
673817
Xanthophyllomyces dendrorhous VKPM Y2410
beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
beta-cryptoxanthin is 3-hydroxy-beta,beta-carotene
-
-
?
1.14.15.24
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
673817
Xanthophyllomyces dendrorhous
zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
zeaxanthin is 3,3'-dihydroxy-beta,beta-carotene
-
-
?
1.14.15.24
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
673817
Xanthophyllomyces dendrorhous VKPM Y2410
zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
zeaxanthin is 3,3'-dihydroxy-beta,beta-carotene
-
-
?
1.14.15.24
beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
673817
Xanthophyllomyces dendrorhous
zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
canthaxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
canthaxanthin is beta,beta-carotene-4-4'-dione
673817
Xanthophyllomyces dendrorhous
phoenicoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
echinenone + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
echinenone is beta,beta-carotene-4-one
673817
Xanthophyllomyces dendrorhous
3-hydroxyechinenone + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
phoenicoxanthin + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
673817
Xanthophyllomyces dendrorhous
astaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
astaxanthin is 3,3'-dihydroxy-beta,beta-carotene-4-4'-dione
-
-
?
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.14.15.24
Xanthophyllomyces dendrorhous
deduced from amino acid sequence
-
5.8