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Literature summary extracted from

  • Worratao, A.; Yongsawatdigul, J.
    Purification and characterization of transglutaminase from tropical tilapia (Oreochromis niloticus) (2005), Food Chem., 93, 651-658.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.2.13 dithiothreitol slight activation at 5 mM Oreochromis niloticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.13 Ba2+ 10 mM, complete inhibition Oreochromis niloticus
2.3.2.13 Cu2+ 10 mM, complete inhibition Oreochromis niloticus
2.3.2.13 EDTA 10 mM, complete inhibition Oreochromis niloticus
2.3.2.13 EGTA 10 mM, complete inhibition Oreochromis niloticus
2.3.2.13 iodoacetic acid 10 mM, complete inhibition Oreochromis niloticus
2.3.2.13 K+ 1 mM, 41% inhibition Oreochromis niloticus
2.3.2.13 Li+ 10 mM, complete inhibition Oreochromis niloticus
2.3.2.13 Mg2+ 1 mM, complete inhibition Oreochromis niloticus
2.3.2.13 N-ethylmaleimide 10 mM, complete inhibition Oreochromis niloticus
2.3.2.13 Na+ 1 mM, 57% inhibition Oreochromis niloticus
2.3.2.13 p-chloromercuribenzoate 10 mM, complete inhibition Oreochromis niloticus
2.3.2.13 phenyl methyl sulfonyl fluoride 10 mM, complete inhibition Oreochromis niloticus
2.3.2.13 Zn2+ 10 mM, complete inhibition Oreochromis niloticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.13 Ca2+ required, optimal concentration 1.25 mM Oreochromis niloticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.13 85000
-
 x * 85000, SDS-PAGE Oreochromis niloticus

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Oreochromis niloticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13
-
 Oreochromis niloticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.13 197
-
 pH 7.5, 37°C Oreochromis niloticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 monodansylcadaverine + N,N-dimethylcasein
-
 Oreochromis niloticus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.13 ? x * 85000, SDS-PAGE Oreochromis niloticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.13 37 50
-
 Oreochromis niloticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.13 7.5
-
-
 Oreochromis niloticus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.2.13 Oreochromis niloticus isoelectric focusing
-
 6.53