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Literature summary extracted from

  • Salo, H.; Sievi, E.; Suntio, T.; Mecklin, M.; Mattila, P.; Renkonen, R.; Makarow, M.
    Co-expression of two mammalian glycosyltransferases in the yeast cell wall allows synthesis of sLex (2005), FEMS Yeast Res., 5, 341-350.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.152 synthesis recombinant yeast cells which provide an inexpensive, self-perpetuating source of fucosyltransferase activity immobilized in the cell wall, useful for in vitro synthesis of sLex Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.152 expression in Pichia pastoris Homo sapiens
2.4.1.152 expression in Saccharomyces cerevisiae Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.152 additional information catalytic domain of alpha-1,3-fucosyltransferase (amino acids 37-441) are expressed in Saccharomyces cerevisiae and Pichia pastoris as a fusion protein HSP150 delta-FucTe consisting of HSP150delta (1-321) and catalytic domain of FucTe (37-441) to promote proper folding and secretion Homo sapiens
2.4.1.152 additional information Saccharomyces cerevisiae strain created co-expressing the catalytic domain of alpha-2,3-sialyltransferase and Fuc-Te in the cell wall, addition of N-acetyllactosamine, CMP-sialic acid and GDP-fucose results in the production of sLex Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.152 1.7
-
 sialyl-alpha-2,3-N-acetyllactosamine fusion protein Hsp150delta-FucTe Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.152 cell wall hsp150delta-FucTe fusion protein remains mostly non-covalently attached to the cell wall Homo sapiens 5618
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.152 MnCl2 glycosyltransferase assay with 20 mM MnCl2 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.152 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.152 GDP-fucose + sialyl-alpha-2,3-N-acetyllactosamine
-
 Homo sapiens GDP + NeuNAcalpha2-3Galbeta1-4[Fucalpha1-3]GlcNAc
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.152 alpha-13-fucosyltransferase VII
-
 Homo sapiens
2.4.1.152 FucTe
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.152 24
-
 glycosyltransferase assay Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.152 7
-
 glycosyltransferase assay Homo sapiens