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Literature summary extracted from

  • Chouayekh, H.; Bejar, W.; Rhimi, M.; Jelleli, K.; Mseddi, M.; Bejar, S.
    Characterization of an l-arabinose isomerase from the Lactobacillus plantarum NC8 strain showing pronounced stability at acidic pH (2007), FEMS Microbiol. Lett., 277, 260-267.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.3.1.4 food industry production of D-tagatose as a low-calorie sugar-substituting sweetener Lactiplantibacillus plantarum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.4 expression in Escherichia coli Lactiplantibacillus plantarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.4 43.4
-
 L-arabinose at 60°C, pH 7.5 Lactiplantibacillus plantarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.4 Co2+ required for maximal activity and stability Lactiplantibacillus plantarum
5.3.1.4 Cu2+ inhibitory Lactiplantibacillus plantarum
5.3.1.4 Mn2+ required for maximal activity and stability Lactiplantibacillus plantarum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.4 55000
-
 SDS-PAGE Lactiplantibacillus plantarum

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.4 Lactiplantibacillus plantarum A9J246 NC8
-
5.3.1.4 Lactiplantibacillus plantarum NC8 A9J246 NC8
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.4 of the recombinant wild type and mutant enzymes Lactiplantibacillus plantarum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.4 263.4
-
 after purification to homogeneity Lactiplantibacillus plantarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.4 D-galactose
-
 Lactiplantibacillus plantarum D-tagatose
-
 ?
5.3.1.4 D-galactose
-
 Lactiplantibacillus plantarum NC8 D-tagatose
-
 ?
5.3.1.4 L-arabinose
-
 Lactiplantibacillus plantarum L-ribulose
-
 ?
5.3.1.4 L-arabinose
-
 Lactiplantibacillus plantarum NC8 L-ribulose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.4 hexamer
-
 Lactiplantibacillus plantarum

Synonyms

EC Number Synonyms Comment Organism
5.3.1.4 D-galactose isomerase
-
 Lactiplantibacillus plantarum
5.3.1.4 L-AI NC8
-
 Lactiplantibacillus plantarum
5.3.1.4 L-arabinose aldose-ketose-isomerase
-
 Lactiplantibacillus plantarum
5.3.1.4 L-arabinose isomerase
-
 Lactiplantibacillus plantarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.4 60
-
-
 Lactiplantibacillus plantarum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.3.1.4 50 65
-
 Lactiplantibacillus plantarum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.4 70
-
 stable for 2 h in the presence of Mn2+ and Co2+ Lactiplantibacillus plantarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.4 7.5
-
-
 Lactiplantibacillus plantarum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.4 6 9
-
 Lactiplantibacillus plantarum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.3.1.4 5 7.5 at 25°C for 50 min Lactiplantibacillus plantarum
5.3.1.4 5
-
 for 24 h Lactiplantibacillus plantarum