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Literature summary extracted from

  • McMahon, B.; Gallagher, M.E.; Mayhew, S.G.
    The protein coded by the PP2216 gene of Pseudomonas putida KT2440 is an acyl-CoA dehydrogenase that oxidises only short-chain aliphatic substrates (2005), FEMS Microbiol. Lett., 250, 121-127.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.8.1 into plasmid pRSET B and overexpressed in Escherichia coli C41(DE3) Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.1 0.02547
-
heptanoyl-CoA
-
Pseudomonas putida
1.3.8.1 0.04231
-
octanoyl-CoA
-
Pseudomonas putida
1.3.8.1 0.09303
-
hexanoyl-CoA
-
Pseudomonas putida
1.3.8.1 0.1238
-
valeryl-CoA
-
Pseudomonas putida
1.3.8.1 0.1253
-
butyryl-CoA
-
Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.8.1 38500
-
4 * 38500, SDS-PAGE, 4 * 40529, amino acid sequence analysis, 4 * 40488, MALDI TOF mass spectroscopy Pseudomonas putida
1.3.8.1 40488
-
4 * 38500, SDS-PAGE, 4 * 40529, amino acid sequence analysis, 4 * 40488, MALDI TOF mass spectroscopy Pseudomonas putida
1.3.8.1 40529
-
4 * 38500, SDS-PAGE, 4 * 40529, amino acid sequence analysis, 4 * 40488, MALDI TOF mass spectroscopy Pseudomonas putida
1.3.8.1 160000
-
gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.1 Pseudomonas putida
-
-
-
1.3.8.1 Pseudomonas putida KT 2240
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.1 by successive fractionations of anion-exchange chromatography, ammonium sulfate precipitation and gel filtration, to more than 99% homogeneity Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.8.1 0.13
-
with hexanoyl-CoA as substrate Pseudomonas putida
1.3.8.1 0.47
-
with 0.21 mM butyryl-CoA as substrate at pH 8.0 Pseudomonas putida
1.3.8.1 0.86
-
with valeryl-CoA as substrate Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.1 butyryl-CoA + electron acceptor
-
Pseudomonas putida 2-butenoyl-CoA + reduced acceptor
-
?
1.3.8.1 butyryl-CoA + electron acceptor
-
Pseudomonas putida KT 2240 2-butenoyl-CoA + reduced acceptor
-
?
1.3.8.1 heptanoyl-CoA + electron acceptor low activity Pseudomonas putida 2-heptenoyl-CoA + reduced acceptor
-
?
1.3.8.1 heptanoyl-CoA + electron acceptor low activity Pseudomonas putida KT 2240 2-heptenoyl-CoA + reduced acceptor
-
?
1.3.8.1 hexanoyl-CoA + electron acceptor
-
Pseudomonas putida 2-hexenoyl-CoA + reduced acceptor
-
?
1.3.8.1 hexanoyl-CoA + electron acceptor
-
Pseudomonas putida KT 2240 2-hexenoyl-CoA + reduced acceptor
-
?
1.3.8.1 additional information fails to oxidize propionyl-CoA, inactive with the CoA derivatives of all phenylalkanoates, the enzyme is not involved in the beta-oxidation of aromatic compounds Pseudomonas putida ?
-
?
1.3.8.1 additional information fails to oxidize propionyl-CoA, inactive with the CoA derivatives of all phenylalkanoates, the enzyme is not involved in the beta-oxidation of aromatic compounds Pseudomonas putida KT 2240 ?
-
?
1.3.8.1 octanoyl-CoA + electron acceptor low activity Pseudomonas putida 2-octenoyl-CoA + reduced acceptor
-
?
1.3.8.1 octanoyl-CoA + electron acceptor low activity Pseudomonas putida KT 2240 2-octenoyl-CoA + reduced acceptor
-
?
1.3.8.1 valeryl-CoA + electron acceptor
-
Pseudomonas putida 2-pentenoyl-CoA + reduced acceptor
-
?

Subunits

EC Number Subunits Comment Organism
1.3.8.1 tetramer 4 * 38500, SDS-PAGE, 4 * 40529, amino acid sequence analysis, 4 * 40488, MALDI TOF mass spectroscopy Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.8.1 0.00183
-
octanoyl-CoA
-
Pseudomonas putida
1.3.8.1 0.00567
-
heptanoyl-CoA
-
Pseudomonas putida
1.3.8.1 0.293
-
butyryl-CoA at pH 5.5 Pseudomonas putida
1.3.8.1 0.2992
-
hexanoyl-CoA
-
Pseudomonas putida
1.3.8.1 0.5883
-
butyryl-CoA at pH 6.0 Pseudomonas putida
1.3.8.1 0.7267
-
butyryl-CoA at pH 8.0 Pseudomonas putida
1.3.8.1 0.73
-
butyryl-CoA at pH 6.5 Pseudomonas putida
1.3.8.1 0.967
-
butyryl-CoA at pH 7.0 Pseudomonas putida
1.3.8.1 0.967
-
butyryl-CoA at pH 7.5 Pseudomonas putida
1.3.8.1 1.743
-
butyryl-CoA
-
Pseudomonas putida
1.3.8.1 2.403
-
valeryl-CoA
-
Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.8.1 7 7.5
-
Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.8.1 5.5 8
-
Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.8.1 FAD one per subunit Pseudomonas putida