EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.8.1 | into plasmid pRSET B and overexpressed in Escherichia coli C41(DE3) | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.8.1 | 0.02547 | - |
heptanoyl-CoA | - |
Pseudomonas putida | |
1.3.8.1 | 0.04231 | - |
octanoyl-CoA | - |
Pseudomonas putida | |
1.3.8.1 | 0.09303 | - |
hexanoyl-CoA | - |
Pseudomonas putida | |
1.3.8.1 | 0.1238 | - |
valeryl-CoA | - |
Pseudomonas putida | |
1.3.8.1 | 0.1253 | - |
butyryl-CoA | - |
Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.8.1 | 38500 | - |
4 * 38500, SDS-PAGE, 4 * 40529, amino acid sequence analysis, 4 * 40488, MALDI TOF mass spectroscopy | Pseudomonas putida |
1.3.8.1 | 40488 | - |
4 * 38500, SDS-PAGE, 4 * 40529, amino acid sequence analysis, 4 * 40488, MALDI TOF mass spectroscopy | Pseudomonas putida |
1.3.8.1 | 40529 | - |
4 * 38500, SDS-PAGE, 4 * 40529, amino acid sequence analysis, 4 * 40488, MALDI TOF mass spectroscopy | Pseudomonas putida |
1.3.8.1 | 160000 | - |
gel filtration | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.8.1 | Pseudomonas putida | - |
- |
- |
1.3.8.1 | Pseudomonas putida KT 2240 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.8.1 | by successive fractionations of anion-exchange chromatography, ammonium sulfate precipitation and gel filtration, to more than 99% homogeneity | Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.8.1 | 0.13 | - |
with hexanoyl-CoA as substrate | Pseudomonas putida |
1.3.8.1 | 0.47 | - |
with 0.21 mM butyryl-CoA as substrate at pH 8.0 | Pseudomonas putida |
1.3.8.1 | 0.86 | - |
with valeryl-CoA as substrate | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.8.1 | butyryl-CoA + electron acceptor | - |
Pseudomonas putida | 2-butenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.1 | butyryl-CoA + electron acceptor | - |
Pseudomonas putida KT 2240 | 2-butenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.1 | heptanoyl-CoA + electron acceptor | low activity | Pseudomonas putida | 2-heptenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.1 | heptanoyl-CoA + electron acceptor | low activity | Pseudomonas putida KT 2240 | 2-heptenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.1 | hexanoyl-CoA + electron acceptor | - |
Pseudomonas putida | 2-hexenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.1 | hexanoyl-CoA + electron acceptor | - |
Pseudomonas putida KT 2240 | 2-hexenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.1 | additional information | fails to oxidize propionyl-CoA, inactive with the CoA derivatives of all phenylalkanoates, the enzyme is not involved in the beta-oxidation of aromatic compounds | Pseudomonas putida | ? | - |
? | |
1.3.8.1 | additional information | fails to oxidize propionyl-CoA, inactive with the CoA derivatives of all phenylalkanoates, the enzyme is not involved in the beta-oxidation of aromatic compounds | Pseudomonas putida KT 2240 | ? | - |
? | |
1.3.8.1 | octanoyl-CoA + electron acceptor | low activity | Pseudomonas putida | 2-octenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.1 | octanoyl-CoA + electron acceptor | low activity | Pseudomonas putida KT 2240 | 2-octenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.1 | valeryl-CoA + electron acceptor | - |
Pseudomonas putida | 2-pentenoyl-CoA + reduced acceptor | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.8.1 | tetramer | 4 * 38500, SDS-PAGE, 4 * 40529, amino acid sequence analysis, 4 * 40488, MALDI TOF mass spectroscopy | Pseudomonas putida |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.8.1 | 0.00183 | - |
octanoyl-CoA | - |
Pseudomonas putida | |
1.3.8.1 | 0.00567 | - |
heptanoyl-CoA | - |
Pseudomonas putida | |
1.3.8.1 | 0.293 | - |
butyryl-CoA | at pH 5.5 | Pseudomonas putida | |
1.3.8.1 | 0.2992 | - |
hexanoyl-CoA | - |
Pseudomonas putida | |
1.3.8.1 | 0.5883 | - |
butyryl-CoA | at pH 6.0 | Pseudomonas putida | |
1.3.8.1 | 0.7267 | - |
butyryl-CoA | at pH 8.0 | Pseudomonas putida | |
1.3.8.1 | 0.73 | - |
butyryl-CoA | at pH 6.5 | Pseudomonas putida | |
1.3.8.1 | 0.967 | - |
butyryl-CoA | at pH 7.0 | Pseudomonas putida | |
1.3.8.1 | 0.967 | - |
butyryl-CoA | at pH 7.5 | Pseudomonas putida | |
1.3.8.1 | 1.743 | - |
butyryl-CoA | - |
Pseudomonas putida | |
1.3.8.1 | 2.403 | - |
valeryl-CoA | - |
Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.8.1 | 7 | 7.5 | - |
Pseudomonas putida |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.3.8.1 | 5.5 | 8 | - |
Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.8.1 | FAD | one per subunit | Pseudomonas putida |