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Literature summary extracted from

  • Foerster-Fromme, K.; Jendrossek, D.
    Malate:quinone oxidoreductase (MqoB) is required for growth on acetate and linear terpenes in Pseudomonas citronellolis (2005), FEMS Microbiol. Lett., 246, 25-31.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.4 gene mqoB, DNA and amino acid sequence determination, analysis, and comparison, expression of mutant enzymes in Escherichia coli strain JM109 Pseudomonas citronellolis
1.1.5.4 gene mqoB, DNA and amino acid sequence determination, analysis, and comparison, expression of mutant enzymes in Escherichia coli strain JM109 Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
1.1.5.4 additional information construction of mqoB mutants by Tn5-Tc transposon insertion, class I mutants show strongly reduced growth on citronellol and citronellic acid, class II mutants grow normally on citronellic acid but reduced on citronellol, class III mutants are auxotroph, overview Pseudomonas citronellolis
1.1.5.4 additional information construction of mqoB mutants by Tn5-Tc transposon insertion, class I mutants show strongly reduced growth on citronellol and citronellic acid, class II mutants grow normally on citronellic acid but reduced on citronellol, class III mutants are auxotroph, overview Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.4 membrane
-
 Pseudomonas aeruginosa 16020
-
1.1.5.4 membrane mainly Pseudomonas citronellolis 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.4 additional information Pseudomonas citronellolis the enzyme is required for growth on acetate and linear terpenes such as citronellol and citronellic acid ?
-
 ?
1.1.5.4 additional information Pseudomonas aeruginosa the enzyme is required for growth on acetate and linear terpenes such as citronellol and citronellic acid ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.4 Pseudomonas aeruginosa Q9HVF1 gene mqoB; gene mqoB
-
1.1.5.4 Pseudomonas citronellolis Q5ECC3 gene mqoB
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.4 (S)-malate + 2,6-dichlorphenolindophenol assay in presence of 2,3-dimethoxy-5-methyl-1,4-benzoquinone Pseudomonas citronellolis oxaloacetate + reduced 2,6-dichlorphenolindophenol
-
 ?
1.1.5.4 (S)-malate + 2,6-dichlorphenolindophenol assay in presence of 2,3-dimethoxy-5-methyl-1,4-benzoquinone Pseudomonas aeruginosa oxaloacetate + reduced 2,6-dichlorphenolindophenol
-
 ?
1.1.5.4 additional information the enzyme is required for growth on acetate and linear terpenes such as citronellol and citronellic acid Pseudomonas citronellolis ?
-
 ?
1.1.5.4 additional information the enzyme is required for growth on acetate and linear terpenes such as citronellol and citronellic acid Pseudomonas aeruginosa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.5.4 malate:quinone oxidoreductase
-
 Pseudomonas citronellolis
1.1.5.4 malate:quinone oxidoreductase
-
 Pseudomonas aeruginosa
1.1.5.4 Mqo
-
 Pseudomonas citronellolis
1.1.5.4 Mqo
-
 Pseudomonas aeruginosa
1.1.5.4 MqoB
-
 Pseudomonas citronellolis
1.1.5.4 MqoB
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.4 30
-
 assay at Pseudomonas citronellolis
1.1.5.4 30
-
 assay at Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.4 7.5
-
 assay at Pseudomonas citronellolis
1.1.5.4 7.5
-
 assay at Pseudomonas aeruginosa