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Literature summary extracted from

  • Ferber, D.M.; Maier, R.M.
    Hydrogen-ubiquinone oxidoreductase activity by the Bradyrhizobium japonicum membrane-bound hydrogenase (1993), FEMS Microbiol. Lett., 110, 257-264.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.5.1 2-n-heptyl-4-hydroxyquinoline-N-oxide 0.1 mM, 17% inhibition Bradyrhizobium japonicum
1.12.5.1 antimycin A 0.1 mM, 47% inhibition Bradyrhizobium japonicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.5.1 Fe a nickel-iron hydrogenase Bradyrhizobium japonicum
1.12.5.1 Ni a nickel-iron hydrogenase Bradyrhizobium japonicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.5.1 33000
-
 1 * 65000 + 1 * 33000 Bradyrhizobium japonicum
1.12.5.1 65000
-
 1 * 65000 + 1 * 33000 Bradyrhizobium japonicum
1.12.5.1 82600
-
 gel filtration Bradyrhizobium japonicum

Organism

EC Number Organism UniProt Comment Textmining
1.12.5.1 Bradyrhizobium japonicum
-
 expresses hydrogenase constitutively
-
1.12.5.1 Bradyrhizobium japonicum SR473
-
 expresses hydrogenase constitutively
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.5.1
-
 Bradyrhizobium japonicum

Subunits

EC Number Subunits Comment Organism
1.12.5.1 dimer 1 * 65000 + 1 * 33000 Bradyrhizobium japonicum